Question About BFB Positive and Cyclic Class Amplicons in AmpliconClassifier

[DinDenkk]

Hi,

Thank you for developing AmpliconClassifier—it has been an invaluable resource for my research. I have a question regarding the interpretation of results in a specific case.

After running AmpliconClassifier, I observed an amplicon that was classified as BFB positive and had an decomposition class of cyclic. I am curious whether such an amplicon can be interpreted as ecDNA based on this classification alone.

I appreciate your clarification or any guidance on this matter and look forward to your insights.

Best regards,