You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
I'm running DropSeqPipe v5.0 with 5 samples in my samples.csv file. However, the pipeline aborted on 1 sample during "rule repair".
After rerunning the pipeline with this sample removed the error occured again on another sample. For the other 3 samples the whole DropSeqPipe pipeline worked fine. Any idea what is happening here? I'm not familiar at all with python and snakemake I'm afraid.
The error:
`Error in rule repair:
jobid: 82
output: ../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz, ../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz
log: ../Results/logs/bbmap/HN4_RNA_repair.txt (check log file(s) for error message)
conda-env: /mnt/go/temp/scRNA-seq/dropSeqPipe/.snakemake/conda/65b8583675236a3c6fd8572f06a27964
shell:
repair.sh -Xmx20g in=../Results/samples/HN4_RNA/trimmed_R1.fastq.gz in2=../Results/samples/HN4_RNA/trimmed_R2.fastq.gz out1=../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz out2=../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz repair=t threads=4 2> ../Results/logs/bbmap/HN4_RNA_repair.txt
(one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)
Removing output files of failed job repair since they might be corrupted:
../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz, ../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz
`
The content of the "/mnt/go/temp/scRNA-seq/Results/logs/bbmap/HN4_RNA_repair.txt" file:
`java -ea -Xmx20g -cp /mnt/go/temp/scRNA-seq/dropSeqPipe/.snakemake/conda/65b8583675236a3c6fd8572f06a27964/opt/bbmap-38.22-1/current/ jgi.SplitPairsAndSingles rp -Xmx20g in=../Results/samples/HN4_RNA/trimmed_R1.fastq.gz in2=../Results/samples/HN4_RNA/trimmed_R2.fastq.gz out1=../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz out2=../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz repair=t threads=4
Executing jgi.SplitPairsAndSingles [rp, -Xmx20g, in=../Results/samples/HN4_RNA/trimmed_R1.fastq.gz, in2=../Results/samples/HN4_RNA/trimmed_R2.fastq.gz, out1=../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz, out2=../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz, repair=t, threads=4]
Set threads to 4
Set INTERLEAVED to false
Started output stream.
/mnt/go/temp/scRNA-seq/dropSeqPipe/.snakemake/conda/65b8583675236a3c6fd8572f06a27964/bin/repair.sh: line 104: 18758 Killed java -ea -Xmx20g -cp /mnt/go/temp/scRNA-seq/dropSeqPipe/.snakemake/conda/65b8583675236a3c6fd8572f06a27964/opt/bbmap-38.22-1/current/ jgi.SplitPairsAndSingles rp -Xmx20g in=../Results/samples/HN4_RNA/trimmed_R1.fastq.gz in2=../Results/samples/HN4_RNA/trimmed_R2.fastq.gz out1=../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz out2=../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz repair=t threads=4
`
The text was updated successfully, but these errors were encountered:
I'm running DropSeqPipe v5.0 with 5 samples in my samples.csv file. However, the pipeline aborted on 1 sample during "rule repair".
After rerunning the pipeline with this sample removed the error occured again on another sample. For the other 3 samples the whole DropSeqPipe pipeline worked fine. Any idea what is happening here? I'm not familiar at all with python and snakemake I'm afraid.
The error:
`Error in rule repair:
jobid: 82
output: ../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz, ../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz
log: ../Results/logs/bbmap/HN4_RNA_repair.txt (check log file(s) for error message)
conda-env: /mnt/go/temp/scRNA-seq/dropSeqPipe/.snakemake/conda/65b8583675236a3c6fd8572f06a27964
shell:
repair.sh -Xmx20g in=../Results/samples/HN4_RNA/trimmed_R1.fastq.gz in2=../Results/samples/HN4_RNA/trimmed_R2.fastq.gz out1=../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz out2=../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz repair=t threads=4 2> ../Results/logs/bbmap/HN4_RNA_repair.txt
(one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)
Removing output files of failed job repair since they might be corrupted:
../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz, ../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz
`
The content of the "/mnt/go/temp/scRNA-seq/Results/logs/bbmap/HN4_RNA_repair.txt" file:
`java -ea -Xmx20g -cp /mnt/go/temp/scRNA-seq/dropSeqPipe/.snakemake/conda/65b8583675236a3c6fd8572f06a27964/opt/bbmap-38.22-1/current/ jgi.SplitPairsAndSingles rp -Xmx20g in=../Results/samples/HN4_RNA/trimmed_R1.fastq.gz in2=../Results/samples/HN4_RNA/trimmed_R2.fastq.gz out1=../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz out2=../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz repair=t threads=4
Executing jgi.SplitPairsAndSingles [rp, -Xmx20g, in=../Results/samples/HN4_RNA/trimmed_R1.fastq.gz, in2=../Results/samples/HN4_RNA/trimmed_R2.fastq.gz, out1=../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz, out2=../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz, repair=t, threads=4]
Set threads to 4
Set INTERLEAVED to false
Started output stream.
/mnt/go/temp/scRNA-seq/dropSeqPipe/.snakemake/conda/65b8583675236a3c6fd8572f06a27964/bin/repair.sh: line 104: 18758 Killed java -ea -Xmx20g -cp /mnt/go/temp/scRNA-seq/dropSeqPipe/.snakemake/conda/65b8583675236a3c6fd8572f06a27964/opt/bbmap-38.22-1/current/ jgi.SplitPairsAndSingles rp -Xmx20g in=../Results/samples/HN4_RNA/trimmed_R1.fastq.gz in2=../Results/samples/HN4_RNA/trimmed_R2.fastq.gz out1=../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz out2=../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz repair=t threads=4
`
The text was updated successfully, but these errors were encountered: