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Spot extraction error when running pipeline with "lsf" profile #46

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cgoina opened this issue Oct 2, 2023 · 0 comments
Open

Spot extraction error when running pipeline with "lsf" profile #46

cgoina opened this issue Oct 2, 2023 · 0 comments
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cgoina commented Oct 2, 2023

Bug report

Description of the problem

Spot extraction fails when running on Janelia's grid with "lsf" profile.
(A clear and concise description of the nature of issue.)

Logs

Command error:
  INFO:    Environment variable SINGULARITYENV_TMPDIR is set, but APPTAINERENV_TMPDIR is preferred
  SCRATCH_DIR: /groups/scicompsoft/home/kawaset/multifish/work/58/d6089cb2ae561627727ffb120b2cf2
  /app/airlocalize/airlocalize.sh /nrs/scicompsoft/kawaset/nftest4/outputs/LHA3_R3_small/stitching/export.n5 /c0/s0 /nrs/scicompsoft/kawaset/nftest4/outputs/LHA3_R3_small/spots/tiles/29/coords.txt /app/airlocalize/params/air_localize_default_params.txt /nrs/scicompsoft/kawaset/nftest4/outputs/LHA3_R3_small/spots/tiles/29 _c0.txt
  Creating MCR_CACHE_ROOT mcr_cache_16
  Running AirLocalize
  Opening N5 from /nrs/scicompsoft/kawaset/nftest4/outputs/LHA3_R3_small/stitching/export.n5
  Reading vox as 'pixelResolution.dimensions' from /c0/s0
  Got vox [0.23 0.23 0.42]
  N5 Path: /nrs/scicompsoft/kawaset/nftest4/outputs/LHA3_R3_small/stitching/export.n5
  N5 Data Set: /c0/s0
  Shape: (429, 1934, 3653)
  Voxel Size: [0.23 0.23 0.42]
  Non-zero voxel count: 64749568
  Initializing MATLAB runtime...
  Traceback (most recent call last):
    File "/app/airlocalize/scripts/air_localize_mcr.py", line 114, in <module>
      AIRLOCALIZE=AIRLOCALIZE_N5.initialize()
    File "/miniconda/lib/python3.6/site-packages/AIRLOCALIZE_N5/__init__.py", line 305, in initialize
      return _pir.initialize_package()
    File "/miniconda/lib/python3.6/site-packages/AIRLOCALIZE_N5/__init__.py", line 253, in initialize_package
      package_handle.initialize()
    File "/usr/local/MATLAB/MATLAB_Runtime/v95/toolbox/compiler_sdk/pysdk_py/matlab_pysdk/runtime/deployablepackage.py", line 33, in initialize
      mcr_handle = self.__cppext_handle.startMatlabRuntimeInstance(self.__ctf_path)
  SystemError: std::exception
  Cleaned up temporary files at mcr_cache_16

Work dir:
  /groups/scicompsoft/home/kawaset/multifish/work/58/d6089cb2ae561627727ffb120b2cf2

Environment

  • EASI-FISH Pipeline version: 1.1.0
  • Nextflow version: 23.04.1.5866
  • Container runtime: Singularity
  • Platform: LSF
  • Operating system: oraclelinux 9
@cgoina cgoina added the bug Something isn't working label Oct 2, 2023
@cgoina cgoina self-assigned this Oct 2, 2023
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