How to generate CARD db

[tianxiahuihui]

In the main.sh, the command "magicblast13 -num_threads $cores -infmt fasta -query $tmp_read_one_trimmed -query_mate $tmp_read_two_trimmed -score 50 -penalty -3 -out $OUT_DIR/$sra.CARD_gene.sam -db $card", but when I download CARD data in https://card.mcmaster.ca/download, I do not know how to generate CARD db by using makeblastdb because
I donot seen a resistance genes fasta file. Coula you tell me how to generate CARD db?

[stevetsa]

Please refer to the updated version of documentation -

wget https://card.mcmaster.ca/download/0/broadstreet-v2.0.0.tar.gz
tar xvf broadstreet-v2.0.0.tar.gz
makeblastdb -in nucleotide_fasta_protein_homolog_model.fasta -dbtype nucl -out cardgenedb
makeblastdb -in nucleotide_fasta_protein_variant_model.fasta -dbtype nucl -out cardsnpdb

https://github.com/stevetsa/MetagenomicAntibioticResistance

[tianxiahuihui]

Thank you for your kidness.