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cannot demultiplex #3

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dridk opened this issue May 15, 2020 · 0 comments
Open

cannot demultiplex #3

dridk opened this issue May 15, 2020 · 0 comments

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@dridk
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dridk commented May 15, 2020 •
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Hi, I don't understand what is wrong ...

I have a fastq file with the following sequences. (My barcode is under bracket):

@MN00591:192:000H332YK:1:11102:20930:2477 1:N:0:1
GCCCCCAAAATCAGCGAAATGGGCGCCATTGGTGATGACACCAGGCATCAGAATAAATGTTGTCGGCGAGATATTTGTCGGTCCCAGACTTTCGCTGAACGGCCACGGAGATAGGGCTAGGAGTAACCAGAATGGAGAACGC  [ATATCATTAACAGAG]  GGTACGCTCATCAGAATTAACC
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

My barcode file contains the barcode :

#filename,#index1,#index2
A11,ATATCATTAACAGAG,  
H10,ATTAATAGTGGTATG,

But the following command keep creating 0 bytes files.

defq -i file.fastq -o demux_out_dir -s index.csv -f .fastq -z 1
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@dridk