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No adapter detected for read and Q20 bases: 4747174600(99.9999%) #557

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h20gg702 opened this issue Apr 8, 2024 · 0 comments
Open

No adapter detected for read and Q20 bases: 4747174600(99.9999%) #557

h20gg702 opened this issue Apr 8, 2024 · 0 comments

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@h20gg702
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h20gg702 commented Apr 8, 2024

Hi, I am analyzing single end RNA-seq data from published source. I am a beginner in this field.
I saw "No adapter detected for read1" and "the Q20 bases: 4747174600(99.9999%)" like below. Is it correct?

/home1/yoshiaki/fastp -i ./SRR23031659.fastq.gz -3 -o out_SRR23031659.fq.gz --html ./report/SRR23031659_fastp.html -j ./report/SRR23031659_fastp.json -q 15 -n 10 -t 1 -T 1 -l 20

Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 47471798
total bases: 4747179800
Q20 bases: 4747174600(99.9999%)
Q30 bases: 4747174600(99.9999%)

Read1 after filtering:
total reads: 47471746
total bases: 4557287616
Q20 bases: 4557287616(100%)
Q30 bases: 4557287616(100%)

Filtering result:
reads passed filter: 47471746
reads failed due to low quality: 52
reads failed due to too many N: 0
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 60.5205%

JSON report: ./report/SRR23031659_fastp.json
HTML report: ./report/SRR23031659_fastp.html

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