Best LibrarySize

[ZitengLiu]

Hi, if my metagenomic paired-end sequencing strategy is 2*150bp, should the LibrarySize in meta-data.txt be 150 or 300? I found that 150 or 300 will result in different 16S sequence numbers.

[biofuture]

Hello, it is 150 Regards, Xiao-Tao Jiang, Ph.D. Postdoc Research Fellow Microbiome Research Centre St George and Sutherland Clinical School UNSW Sydney Level 2, Clinical Sciences Building (WR Pitney) Short Street, St George Hospital KOGARAH NSW 2217 T: +61 402 943 681 Email: ***@***.******@***.*** MRC web: https://microbiome.org.au/ liuziteng1006 ***@***.***> 于2021年8月14日周六 上午2:06写道：

…

Hi, if my metagenomic paired-end sequencing strategy is 2*150bp, should the LibrarySize in meta-data.txt be 150 or 300? I found that 150 or 300 will result in different 16S sequence numbers. — You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub <#63>, or unsubscribe <https://github.com/notifications/unsubscribe-auth/AALLDIFIZLT6IYFN3XJZ3XDT4U7INANCNFSM5CD3W34Q> . Triage notifications on the go with GitHub Mobile for iOS <https://apps.apple.com/app/apple-store/id1477376905?ct=notification-email&mt=8&pt=524675> or Android <https://play.google.com/store/apps/details?id=com.github.android&utm_campaign=notification-email> .