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Xenome classify output file #32

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ssinghk opened this issue Aug 8, 2021 · 2 comments
Open

Xenome classify output file #32

ssinghk opened this issue Aug 8, 2021 · 2 comments

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@ssinghk
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ssinghk commented Aug 8, 2021

Hello,
I am using xenome classify to process xenograft RNA-seq data. The output species specific .fastq files are missing '@' or '>' and the STAR aligner is unable to map.

@RamRS
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RamRS commented Mar 3, 2022

Which version are you using? I see this bug in xenome version 1.0.1 downloaded from #9 (comment)

I use sed '1~4s/^/@/' each_output.fastq to get around it.

@dgomezpere
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Hi,

I would also suggest to introduce a + in the 3rd line of each read with sed -i 's/^$/+/' each_output.fastq.
I found some problems in downstream analyses using the "FASTQ" format from Xenome.

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