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Sorry for Bothering again, but after testing on my entire dataset not just 1 sample, around 3/4 million cell, i observe this MAP projection and am not sure what could be the issue while doing same exact worflow on just 1 libarary the projection looks normal (11k cells) or when subsetting the same adata object to few number of cells.
here is the code and projection below,
adata=sc.read_h5ad("/pasteur/zeus/projets/p02/LabExMI/singleCell/V3/scRNA_NS_IAV_COV/results/merged_object/adata_scvi.h5ad")
adata.X=adata.X.toarray()
sc.pp.scale(adata, max_value=10)
adata = adata[:, adata.var.highly_variable]
tg = tp.TopOGraph(n_eigs=150, n_jobs=-1, verbosity=0)
tg.run_models(adata.X, kernels=['bw_adaptive'],
eigenmap_methods=['DM'],
projections=['MAP'])
tg
TopOGraph object with 727581 samples and 12855 observations and:
. Base Kernels:
bw_adaptive - .BaseKernelDict['bw_adaptive']
. Eigenbases:
DM with bw_adaptive - .EigenbasisDict['DM with bw_adaptive']
. Graph Kernels:
bw_adaptive from DM with bw_adaptive - .GraphKernelDict['bw_adaptive from DM with bw_adaptive']
. Projections:
MAP of bw_adaptive from DM with bw_adaptive - .ProjectionDict['MAP of bw_adaptive from DM with bw_adaptive']
Active base kernel - .base_kernel
Active eigenbasis - .eigenbasis
Active graph kernel - .graph_kernel
adata.obsm['X_topoMAP'] = tg.ProjectionDict['MAP of bw_adaptive from DM with bw_adaptive']
The text was updated successfully, but these errors were encountered:
Sorry for Bothering again, but after testing on my entire dataset not just 1 sample, around 3/4 million cell, i observe this MAP projection and am not sure what could be the issue while doing same exact worflow on just 1 libarary the projection looks normal (11k cells) or when subsetting the same adata object to few number of cells.
here is the code and projection below,
The text was updated successfully, but these errors were encountered: