diff --git a/tox.ini b/tox.ini
index aceac8742c6d..a78ac2f28f00 100644
--- a/tox.ini
+++ b/tox.ini
@@ -1,7 +1,21 @@
 [tox]
 # envlist is the list of environments that are tested when `tox` is run without any option
 # hyphens in an environment name are used to delimit factors
-envlist = py37-first_startup, py37-lint, py37-lint_docstring_include_list, py37-mypy, py37-reports_startup, py37-unit, test_galaxy_packages, validate_test_tools
+envlist =
+    py{37,38,39,310,311}-first_startup
+    py{37,38,39,310,311}-lint
+    py{37,38,39,310,311}-lint_docstring_include_list
+    py{37,38,39,310,311}-mypy
+    py{37,38,39,310,311}-reports_startup
+    py{37,38,39,310,311}-unit
+    first_startup
+    lint
+    lint_docstring_include_list
+    mypy
+    reports_startup
+    unit
+    test_galaxy_packages
+    validate_test_tools
 skipsdist = True
 
 [testenv]