genome size

[jorgeibarracaballero]

Hello.
I want to try your program but before going into installing it and trying to actually try to use it, I want to ask you if there is a limit for the size of the genomes that the program can handle. I work with fungal genomes and they are very big compared to bacteria. Also, they the genome assemblies are made of many contigs and I wonder if that will work. Thank you

[jiqingxiaoxi]

Hi,
1)The input fasta file must be only one sequence. So you should split your genomes into several files, each file contains one sequence/genome/contig.
2)GLAPD can design primers for very large genome if your server has enough memory. But it will take a long time to run GLAPD. You can split your sequence into small fragment(for example 50Mb/fragment) to accelerate the design.

Best wishes.