Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Call input and runtime attributes evaluation failed for PairedFastQsToUnmappedBAM #644

Open
Ananya-swi opened this issue Apr 21, 2023 · 0 comments

Comments

@Ananya-swi
Copy link

Hi,

I am trying to run the Example Workflow: Convert FASTQ files to uBAM file using Cromwell On Azure.

I used the publicly available input file URLs and uploaded into the "inputs" container in Cromwell on Azure storage account "demo0fd990508f1745" associated with the host VM.
While running the example workflow, I got the below error message:

{
  "WorkflowUrl": "https://demo0fd990508f1745.blob.core.windows.net/inputs/paired-fastq-to-unmapped-bam.wdl",
  "WorkflowInputsUrl": "https://demo0fd990508f1745.blob.core.windows.net/inputs/paired-fastq-to-unmapped-bam.inputs.json",
  "WorkflowInputsUrls": null,
  "WorkflowOptionsUrl": null,
  "WorkflowDependenciesUrl": null,
  "WorkflowFailureInfo": {
    "WorkflowFailureReason": "CromwellFailed",
    "CromwellFailureLogs": [
      {
        "Message": "Workflow failed",
        "CausedBy": [
          {
            "Message": "Call input and runtime attributes evaluation failed for PairedFastQsToUnmappedBAM",
            "CausedBy": [
              {
                "Message": "Failed to evaluate input 'disk_space_gb' (reason 1 of 1): [Attempted 1 time(s)] - NoSuchFileException: /demo0fd990508f1745/inputs/seq-format-conversion/NA12878_20k/H06HDADXX130110.1.ATCACGAT.20k_reads_1.fastq",
                "CausedBy": []
              }
            ]
          }
        ]
      }
    ]
  }
}

The Used inputs.json file is the below one.

{
  "ConvertPairedFastQsToUnmappedBamWf.readgroup_name": "NA12878_A",
  "ConvertPairedFastQsToUnmappedBamWf.sample_name": "NA12878",
  "ConvertPairedFastQsToUnmappedBamWf.fastq_1": "/demo0fd990508f1745/inputs/seq-format-conversion/NA12878_20k/H06HDADXX130110.1.ATCACGAT.20k_reads_1.fastq",
  "ConvertPairedFastQsToUnmappedBamWf.fastq_2": "/demo0fd990508f1745/inputs/seq-format-conversion/NA12878_20k/H06HDADXX130110.1.ATCACGAT.20k_reads_2.fastq", 
  "ConvertPairedFastQsToUnmappedBamWf.library_name": "Solexa-NA12878",
  "ConvertPairedFastQsToUnmappedBamWf.platform_unit": "H06HDADXX130110.2.ATCACGAT",
  "ConvertPairedFastQsToUnmappedBamWf.run_date": "2016-09-01T02:00:00+0200",
  "ConvertPairedFastQsToUnmappedBamWf.platform_name": "illumina",
  "ConvertPairedFastQsToUnmappedBamWf.sequencing_center": "BI",
  "ConvertPairedFastQsToUnmappedBamWf.make_fofn": true  
}

The same example wdl file is used for processing (https://github.com/microsoft/seq-format-conversion-azure/blob/main-azure/paired-fastq-to-unmapped-bam.wdl) along with the trigger json file.

Can you help me understand the issue (the input fastq files are available inside the folder)?

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant