diff --git a/modules/local/dreg_prep/templates/proseq2.0 b/modules/local/dreg_prep/templates/proseq2.0
index 3ca2f0bf..f55d0d04 100755
--- a/modules/local/dreg_prep/templates/proseq2.0
+++ b/modules/local/dreg_prep/templates/proseq2.0
@@ -70,7 +70,7 @@ readCount=`zcat ${prefix}.bed.gz | wc -l`
 echo \${readCount}
 
 ## Remove rRNA and reverse the strand (PRO-seq).
-zcat ${prefix}.bed.gz | grep "rRNA\\|chrM" -v | grep "_" -v | sort -k 1,1 -k2,2n - | gzip >${prefix}.nr.rs.bed.gz
+zcat ${prefix}.bed.gz | grep "rRNA\\|chrM" -v | sort -k 1,1 -k2,2n - | gzip >${prefix}.nr.rs.bed.gz
 echo 'Number of mappable reads (excluding rRNA):'
 echo `zcat ${prefix}.nr.rs.bed.gz | wc -l`