diff --git a/samples/bluetooth/peripheral_uart/Kconfig.sysbuild b/samples/bluetooth/peripheral_uart/Kconfig.sysbuild
index d56170702508..9f7d7017d591 100644
--- a/samples/bluetooth/peripheral_uart/Kconfig.sysbuild
+++ b/samples/bluetooth/peripheral_uart/Kconfig.sysbuild
@@ -6,8 +6,19 @@
 
 source "${ZEPHYR_BASE}/share/sysbuild/Kconfig"
 
+config NRF_IPC_RADIO_RPC_HOST
+	bool "Enable building RPC Host for Radio domain"
+	help
+	  The option enables building sample rpc_host application for Radio
+	  domain. This sysbuild configuration option should be selected only if
+	  an Application core application is build with CONFIG_BT_RPC_STACK
+	  enabled.
+
 config NRF_DEFAULT_IPC_RADIO
 	default y
 
 config NETCORE_IPC_RADIO_BT_HCI_IPC
-	default y
+	default y if !NRF_IPC_RADIO_RPC_HOST
+
+config NRF_DEFAULT_RPC_HOST
+	default y if NRF_IPC_RADIO_RPC_HOST
diff --git a/samples/bluetooth/peripheral_uart/sample.yaml b/samples/bluetooth/peripheral_uart/sample.yaml
index ee028106af8d..3915ac2d006e 100644
--- a/samples/bluetooth/peripheral_uart/sample.yaml
+++ b/samples/bluetooth/peripheral_uart/sample.yaml
@@ -47,7 +47,18 @@ tests:
       - CONFIG_BT_RPC_STACK=y
     integration_platforms:
       - nrf5340dk/nrf5340/cpuapp
-    platform_allow: nrf5340dk/nrf5340/cpuapp
+      - nrf54h20dk/nrf54h20/cpuapp
+    platform_allow: nrf5340dk/nrf5340/cpuapp nrf54h20dk/nrf54h20/cpuapp
+    tags: bluetooth ci_build sysbuild
+  sample.bluetooth.peripheral_uart_ble_rpc.54h20:
+    sysbuild: true
+    build_only: true
+    extra_configs:
+      - SNIPPET=nordic-rpc-host
+      - SB_CONFIG_NRF_IPC_RADIO_RPC_HOST=y
+    integration_platforms:
+      - nrf54h20dk/nrf54h20/cpuapp
+    platform_allow: nrf54h20dk/nrf54h20/cpuapp
     tags: bluetooth ci_build sysbuild
   sample.bluetooth.peripheral_uart.security_disabled:
     sysbuild: true