diff --git a/samples/peripheral/lpuart/README.rst b/samples/peripheral/lpuart/README.rst
index f8a332a8962a..f6ad1aa5c38b 100644
--- a/samples/peripheral/lpuart/README.rst
+++ b/samples/peripheral/lpuart/README.rst
@@ -20,8 +20,34 @@ The sample supports the following development kits:
 
 The sample also requires the following pins to be shorted:
 
-* TX (Arduino Digital Pin 10 (4 on nRF21540 DK)) with RX (Arduino Digital Pin 11 (5 on nRF21540 DK))
-* Request Pin (Arduino Digital Pin 12 (6 on nRF21540 DK)) with Response Pin (Arduino Digital Pin 13 (7 on nRF21540 DK))
+   .. list-table:: Pin connections.
+      :widths: auto
+      :header-rows: 1
+
+      * - Development Kit
+        - nRF52 DK
+        - nRF52833 DK
+        - nRF52840 DK
+        - nRF21540 DK
+        - nRF5340 DK pins
+        - nRF54L15 DK pins
+        - nRF9160 DK pins
+      * - Request-Response Pins
+        - P0.24-P0.25
+        - P0.22-P0.23
+        - P1.14-P1.15
+        - P1.07-P1.08
+        - P1.14-P1.15
+        - P1.08-P1.09
+        - P0.12-P0.13
+      * - UART RX-TX Pins
+        - P0.22-P0.23
+        - P0.20-P0.21
+        - P1.12-P1.13
+        - P1.05-P1.06
+        - P1.12-P1.13
+        - P1.10-P1.11
+        - P0.10-P0.11
 
 Additionally, it requires a logic analyzer.
 
diff --git a/samples/peripheral/lpuart/boards/nrf21540dk_nrf52840.overlay b/samples/peripheral/lpuart/boards/nrf21540dk_nrf52840.overlay
index 64a0726dc974..5e078327314a 100644
--- a/samples/peripheral/lpuart/boards/nrf21540dk_nrf52840.overlay
+++ b/samples/peripheral/lpuart/boards/nrf21540dk_nrf52840.overlay
@@ -19,11 +19,11 @@
 	 * observed on nRF21540 v2.0.0 only (~450 uA more than on v1.0.0).
 	 */
 	group1 {
-		psels = <NRF_PSEL(UART_TX, 0, 20)>,
+		psels = <NRF_PSEL(UART_TX, 0, 6)>,
 			<NRF_PSEL_DISCONNECTED(UART_RTS)>;
 	};
 	group2 {
-		psels = <NRF_PSEL(UART_RX, 0, 22)>,
+		psels = <NRF_PSEL(UART_RX, 0, 8)>,
 			<NRF_PSEL_DISCONNECTED(UART_CTS)>;
 	};
 };
diff --git a/samples/peripheral/lpuart/boards/nrf52840dk_nrf52840.overlay b/samples/peripheral/lpuart/boards/nrf52840dk_nrf52840.overlay
index 3607890b53e6..773f95138f87 100644
--- a/samples/peripheral/lpuart/boards/nrf52840dk_nrf52840.overlay
+++ b/samples/peripheral/lpuart/boards/nrf52840dk_nrf52840.overlay
@@ -23,11 +23,11 @@
 	 * observed on nRF52840 v3.0.0 only (~450 uA more then on v2.1.0).
 	 */
 	group1 {
-		psels = <NRF_PSEL(UART_TX, 0, 20)>,
+		psels = <NRF_PSEL(UART_TX, 0, 6)>,
 			<NRF_PSEL_DISCONNECTED(UART_RTS)>;
 	};
 	group2 {
-		psels = <NRF_PSEL(UART_RX, 0, 22)>,
+		psels = <NRF_PSEL(UART_RX, 0, 8)>,
 			<NRF_PSEL_DISCONNECTED(UART_CTS)>;
 	};
 };