Annotate update coordinates

[Glfrey]

Hello again!

I just noticed when I do some annotation updates like so:

metagraph annotate -i Chr1.dbg -o seperate -v --anno-filename --coordinates -a seperate.column.annodbg SpA_Chr1.fa

The *annodbg data structure gets updated but the *.annodbg.coords gets overwritten so only one sequences coordinates is being stored. Is there any way to iteratively update the coordinate file? Or do I have to re-do all coordinate annotations every time I want to add a new sequence to the graph?

[hmusta]

Sorry for responding late. Every time you add a sequence to the graph, it changes the coordinate system, so you need to reannotate the graph.

[Glfrey]

Hi @hmusta, thanks for getting back to me, that makes sense. I was trying to update the graph iteratively as I have a load of very large sequences to annotate with coordinates, what would you suggest is the most memory efficient way of doing this? So far I have --mem-cap-gb 0.001 and --disk-swap. Is there anything else?