How to load .Robj file

[rishirajmd]

Hello, a very basic question. How do I load the Seurat .Robj files downloaded from Figshare. Thanks. Rishi.

[timoast]

You can use the load() function in R to load objects that were saved using the save() function, or use readRDS() for those saved using saveRDS().

[rishirajmd]

Thank you. The load(). Worked. RR.

[strawberry098]

After loading the .Robj file (ref) downloaded from Figshare, I want to integrate it with an .rds file (slide.seq) for label transfer:

anchors <- [FindTransferAnchors](https://satijalab.org/seurat/reference/FindTransferAnchors.html)(reference = ref, query = slide.seq, normalization.method = "SCT", npcs = 50)

but I get the following error:

Error in UseMethod(generic = "DefaultAssay", object = object) :
no applicable method for 'DefaultAssay' applied to an object of class "seurat"