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Hi, We used two different single cell methods (10X and SMARTseq) on the same tissue and first of all, I analysed both datasets separately. Then I used Harmony to integrate them. Now I would like to label cells based on where they clustered in the separate analyses. For instance, I would like to see in which cluster cells from cluster 8 in the SMARTseq- or cluster 10 in the 10X-dataset end up when both are integrated. I know that I can extract cell IDs using "WhichCells" and that I can add information to a Seurat object using "AddMetaData". Do I have to combine these two in some way? Many thanks in advance! Best, |
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Please see the label transfer vignettes on the Seurat website: https://satijalab.org/seurat/articles/integration_mapping.html |
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Please see the label transfer vignettes on the Seurat website: https://satijalab.org/seurat/articles/integration_mapping.html