Help! Volcano plot in R on scRNAseq data

[scRNAseqnovice]

Hi, I am a novice in analyzing scRNAseq data. I have been following the Satija lab tutorials and have found them intuitive and useful so far.

I would like to create a volcano plot to compare differentially expressed genes (DEGs) across two samples- a "before" and "after" treatment.

I have scoured the web but I still cannot figure out how to do this.

I have successfully installed ggplot, normalized my datasets, merged the datasets, etc., but what I do not understand is how to transfer the sequencing data to the ggplot function. I keep receiving an error that says:

"data must be a <data.frame>, or an object coercible by fortify(), not an S4 object with class ."

I have seen tutorials on the web, but the data there is not processed the same as how I have been doing following the Satija lab method, and, my files are not .csv, but instead are .tsv

Any tips would be appreciated!

Thanks!