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I have Seurat v5.0.1 installed on two different desktops (same specs, just different machines) and I was able to successfully run through an entire analysis of a dataset using Seurat v5 pipeline with BPCells on Computer A and saved the Seurat object. However, reading in the same object on Computer B, it loads in correctly, but doing something as simple as running the NormalizeData function gives the following error:
Normalizing layer: counts
Error in h(simpleError(msg, call)) :
error in evaluating the argument 'x' in selecting a method for function 't': no slot of name "threads" for this object of class "ColBindMatrices"
Attached are the results of running sessionInfo() on both computers. After checking the differences between the two, Computer A (Seurat v5 works) has slightly older versions of cluster, KernSmooth, lattice, MASS, nlme, & survival with presto_1.0.0 and rstudioapi_0.15.0 loaded in the namespace as extra. Computer B has the slightly newer versions of those packages listed previously and the addition of BiocGenerics_0.48.1, bitops_1.0-7, GenomeInfoDb_1.38.5, GenomeInfoDbData_1.2.11, GenomicRanges_1.54.1, IRanges_2.36.0, RCurl_1.98-1.14, S4Vectors_0.40.2, stats4_4.3.1, XVector_0.42.0, & zlibbioc_1.48.0. I don't want to mess around on Computer A and update things to match Computer B and then lose all functionality and access to my Seurat objects, so if anyone has any information that could indicate why this error is occurring, I would appreciate the help.
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I have Seurat v5.0.1 installed on two different desktops (same specs, just different machines) and I was able to successfully run through an entire analysis of a dataset using Seurat v5 pipeline with BPCells on Computer A and saved the Seurat object. However, reading in the same object on Computer B, it loads in correctly, but doing something as simple as running the NormalizeData function gives the following error:
Normalizing layer: counts
Error in h(simpleError(msg, call)) :
error in evaluating the argument 'x' in selecting a method for function 't': no slot of name "threads" for this object of class "ColBindMatrices"
Attached are the results of running sessionInfo() on both computers. After checking the differences between the two, Computer A (Seurat v5 works) has slightly older versions of cluster, KernSmooth, lattice, MASS, nlme, & survival with presto_1.0.0 and rstudioapi_0.15.0 loaded in the namespace as extra. Computer B has the slightly newer versions of those packages listed previously and the addition of BiocGenerics_0.48.1, bitops_1.0-7, GenomeInfoDb_1.38.5, GenomeInfoDbData_1.2.11, GenomicRanges_1.54.1, IRanges_2.36.0, RCurl_1.98-1.14, S4Vectors_0.40.2, stats4_4.3.1, XVector_0.42.0, & zlibbioc_1.48.0. I don't want to mess around on Computer A and update things to match Computer B and then lose all functionality and access to my Seurat objects, so if anyone has any information that could indicate why this error is occurring, I would appreciate the help.
ComputerA_SessionInfo.txt
ComputerB_SessionInfo.txt
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