Replies: 1 comment
-
Circling back to address the issue of removing gene symbols from RNA-seq datasets containing clones are long coding RNAs that are undesirable for downstream analysis. So far, the easiest way that I found to do this is to 1) save a DGE analysis as a csv, 2) open in Excel (do not convert) and sort the data by the feature name in alphabetical order, and 3) delete the rows with symbols beginning with AC, AF, AJ, AL, AP, FP (and LINC if they are not needed). They are easy to spot in groups and the task is relatively painless if you do not have a high volume of datasets. |
Beta Was this translation helpful? Give feedback.
0 replies
Sign up for free
to join this conversation on GitHub.
Already have an account?
Sign in to comment
-
RNA-seq data often annotate features that are not defined as genes per se and have been listed with an accession prefix + clone or long coding RNA number. These can influence downstream enrichment analyses for function and cell types. Is there an easy way to search and remove them rather than doing it manually? I've searched around a bit for methods but so far I have not come across any.
Beta Was this translation helpful? Give feedback.
All reactions