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I got the answer, just use |
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I am following the Seurat - Guided Clustering Tutorial and I am trying to reproduce results obtained from nFeature_RNA and nCount_RNA by my self. Next I provide the code I am working on
So as stated here for example, nFeature_RNA is just the number of features expressed in a given cell and nCount_RNA is the number of molecules expressed in a given cell. That would be just counting the number of genes with values above zero and the sum of values over all genes for a given set. That is the calculations I am doing but numbers do not match with those stored in seurat object. Am I missing something?
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