very large h5seurat file

[Rajesh-HKU]

Dear all,
I have downloaded a very large h5seuratfile (~30gb) in size. How do I read it? Loadh5Seurat fails due to memory size.
Thanks in advance.

Best regards,
Rajesh

[Rajesh-HKU]

I converted the only the RNA assay from the h5seurat file to h5ad using SeuratDisk::Convert and then used BPCells::open_matrix_hdf5 to open the converted file. I used BPCells::write_matrix_dir to save it in on disk. Subsequently open_matrix_dir followed by standard Seurat pipeline.

key step was to abandon the SCTassay in the first step