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I have multiple samples that I integrated using SCTransform and RPCA integration. After subsetting a portion of the cells from the integrated object, do I need to re-run SCTransform on the subsetted cells? Also, do I need to re-run PrepSCTFindMarkers? Any advice would be greatly appreciated!
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Hello,
I have multiple samples that I integrated using SCTransform and RPCA integration. After subsetting a portion of the cells from the integrated object, do I need to re-run SCTransform on the subsetted cells? Also, do I need to re-run PrepSCTFindMarkers? Any advice would be greatly appreciated!
Thank you!
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