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I'm working on scRNA-seq analysis pipeline using Seurat. On Ch. 7: https://www.singlecellcourse.org/biological-analysis.html, those clustering examples are very useful! The only problem is that in DimPlot, they only have "tsne," "pca" and "umap" as 'reduction' options. Not only that, but I want to try Hierarchical Clustering, K-means and "Graph-based Methods." But, I cannot see much coding examples with them. Just figures and texts about them. They do show a little coding how to get tSNE, PCA and heatmap. But otherwise, how do you set up coding to get graphical visualization/plots of 1) Hierarchical Clustering heatmap, 2) k-means clustering and 3) graph-based clustering?
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Hey Everyone,
I'm working on scRNA-seq analysis pipeline using Seurat. On Ch. 7: https://www.singlecellcourse.org/biological-analysis.html, those clustering examples are very useful! The only problem is that in DimPlot, they only have "tsne," "pca" and "umap" as 'reduction' options. Not only that, but I want to try Hierarchical Clustering, K-means and "Graph-based Methods." But, I cannot see much coding examples with them. Just figures and texts about them. They do show a little coding how to get tSNE, PCA and heatmap. But otherwise, how do you set up coding to get graphical visualization/plots of 1) Hierarchical Clustering heatmap, 2) k-means clustering and 3) graph-based clustering?
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