12-materials.Rmd

# Solutions and Materials{#mm}
## Solutions{#solutions}
### Label buffer{#washingbuffer}

Materials:

* ddH2O (500 ml)
* 140 mM NaCl (4.1 g)
* 5 mM Hepes (0.569 g)
* pH calibration 7.4

Procedure:

* Weigh the correct amounts of Hepes and NaCl
* Resolve in a glas bottle with 450 ml of water
* Stir carefully
* Check and adjust pH
* Adjust volumes to 500 ml

### MCW{#mcw}

Materials:

* Methanol
* Chloroform
* ddH2O
* Cinnamic acid stock in MeOH (2 mg/ml): final conc. 2 ug/ml

Procedure:

* Mix the solvents in the ratio of volumes - Methanol:Chlorofom:Water -- 5:2:1
* Supplement cinnamic acid stock 1:1000
* Store at -25°C

### Alkane-Mix{#alkanemix}

Materials:

* Hexane
* Alkanes: c10, c12, c15, c17, c19, c22, c28, c32, c36
* Thermo mixer
* Glass vials and caps

Procedure:

* Prepare stock solutions in hexane:
  + c10 - c17 (liquid): 25 ul/ml
  + c19 - c32: 20 mg/ml
  + c36: two-times 15 mg/1.5 ml
* Warm up alkane stocks in thermo mixer 40°C
* Prepare a text mixture in equal amounts, e.g., 50 ul each, but use twice the volume of c36
* Mix test mixture with MSTFA: 10 ul / 1 ml MSTFA
* Check alkane profile by GC-MS
* If required: adjust volumes and re-test or create larger volume of zour mixture for aliquots
* Store aiquots in glass vials, close well and store at 4°C
* For usage: gently warm up glass vials at 30 C on thermo mixer for 10 min and vortex before adding it to the MSTFA

Adjust the volumes of the alkane stocks in order to create a curve shaped distribution of all alkanes in the chromatogram: lower intensities for c10 and c32-36, slowly increasing intensities for the alkanes in between.


## Materials

```{r chemicals,echo=FALSE}

chem = read.csv("tables/chemicals.csv", TRUE)

knitr::kable(chem, bookstab = TRUE, caption = "List of chemicals including company names and catalog number.")
```