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add Maize apollo curated gene set to gramene browser #12

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weix-cshl opened this issue May 10, 2019 · 3 comments
Open

add Maize apollo curated gene set to gramene browser #12

weix-cshl opened this issue May 10, 2019 · 3 comments

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@weix-cshl
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The community apollo curation project manually curated 109 maize gene models and generated the improved model as a GFF file, which can be attach as a custom track in Gramene's genome browser.
In the mean time, we want to served them as a permanent track. To do it, we need to load the models to maize otherfeatures database.

As a test, I loaded them to the database of last release and displayed them on the previous browser (Grm60). Here is the an example:
Zeamays_326812832772776

http://paulaner.cshl.edu/Zea_mays/Location/View?r=3:2681283-2772776;db=core

@weix-cshl
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We missed ensemblgenome freeze deadline for June release (e97,eg44), because we found some data issue and had to clean the gff3 file up. But this also gave us more time to make sure our data is correct and our displaying strategy works.

We haven't got the protein sequence to validate the loaded models yet.

  1. ftp://ftp.gramene.org/pub/gramene/CURRENT_RELEASE/gff3/zea_mays/curated_apollo_annotations_maize_v4.gff3
    The gff3 was validated at
    http://genometools.org/cgi-bin/gff3validator.cgi

  2. working directory
    brie:/home/weix/data/genomes/Zea_mays_AGPv4/ApolloCurated>

tested on colden:zea_mays_otherfeatures_60_95_7
perl ./load_genes_withAEDmultiP_from_gff3.pl -e ./ZmGrm60.registry -s zea_mays -l curated_genes curated_apollo_annotations_maize_v4.gff3

add entry to analysis_description in the otherfeatures database.

cat analysis_description.apollo 
76      Manually curated AGPv4 gene models with Apollo Annotation Editor        curated_apollo_annotations      1       {'multi_name' => 'curated_apollo_annotations','colour_key' => '[biotype]','caption' => 'curated_apollo_annotations','name' => 'Manually curated AGPv4 gene models with Apollo','label_key' => '[text_label] [display_label]','default' => {'MultiTop' => 'gene_label','contigviewbottom' => 'transcript_label','MultiBottom' => 'collapsed_label','contigviewtop' => 'gene_label','cytoview' => 'gene_label','alignsliceviewbottom' => 'as_collapsed_label'},'key' => 'apollo_curation'}

@weix-cshl
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Validated 269 proteins, loaded the 123 genes (271 transcripts) into live site database:
colden:zea_mays_otherfeatures_61_96_7.

Here is a example
http://ensembl.gramene.org/Zea_mays/Location/View?r=3:2707671-2714923;db=core
Zeamays_32707671_2714923

@marcelakarey
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Looks great. Thank you, Sharon!

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