We have developed a bioinformatics workflow that could reliably characterise sequences from small-RNA sequencing to different databases of small non-coding RNAs, and more precisely tackle the crucial issue regarding the piRNA annotation. You can find the published article here: f1000
The workflow consists of three significant steps:
- Annotation forging: the generation of the annotation files for small RNA sequences used in the next quantification step.
- Pre-processing & Quantification: pre-processing, alignment and quantification of the reads assigned to sncRNAs (using a dual approach: genomic and transcriptomic analysis).
- Exploratory data analysis: result exploration of both quantification methods in parallel and Differential Expression (DE) with two different methodologies (edgeR and limma-voom).
| Logo | workflow |
|---|---|
 |
 |