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wind: wORKFLOW FOR PiRNAs AnD BEYONd

DOI

We have developed a bioinformatics workflow that could reliably characterise sequences from small-RNA sequencing to different databases of small non-coding RNAs, and more precisely tackle the crucial issue regarding the piRNA annotation. You can find the published article here: f1000

The workflow consists of three significant steps:

  1. Annotation forging: the generation of the annotation files for small RNA sequences used in the next quantification step.
  2. Pre-processing & Quantification: pre-processing, alignment and quantification of the reads assigned to sncRNAs (using a dual approach: genomic and transcriptomic analysis).
  3. Exploratory data analysis: result exploration of both quantification methods in parallel and Differential Expression (DE) with two different methodologies (edgeR and limma-voom).
Logo workflow