Quality Control concepts of Next Generation Sequencing with Illumina

Undertand and interpret the main quality control of the analisys of the raw data with fastqc

About the course

This course is address to researchers with not prior experience in bioinformatics, they will be able to interpret the main parameters of FASTQC quality control tool for short reads (Illumina) and known the main tools for remove low quality reads and adapater content.

Outline

Learning objectives

• Execute/Run FASTQC on short reads obtained by illumina sequencing.
• Interpret the Quality Control Parameters offered by FASTQC
• Understand the sequencing technology of Illumina.
• Main factors for choose an appropiate library preparation kit
• Description of the main tools for low quality reads and adapter removal.

Prerequisite / technical requirements

Being comfortable working with the CLI (command-line interface) in a Linux-based environment.

Acknowledgements

• Sphinx. The publication system for our course pages.