FlowCytSpeed

R Script to plot the average event rate in flow or mass cytometry (FCS) files.

Description

As per the description, I created this script as a "QC" step to examine the rate of flow of cells (flow/mass/CyTOF) and/or events and cells (mass/CyTOF) on a flow or mass (CyTOF) cytometer.

I got the idea from another package, FlowAI. That package could (and should?) be used to cleanup any data that has big spikes in the flow rate.

Usage

Copy / paste and run the script in R and perhaps in an IDE such as R-Studio

Example Output

Flow

Mass (CyTOF)
This is quite an interesting dataset because it's been concatenated and also shows all events (blue) as well as cell events (red). There must have been a huge spike in contamination or some other non-cell material about halfway through.

Issues

Please report any bugs / suggestions here.