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kapoorlab committed Sep 3, 2023
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2 changes: 1 addition & 1 deletion README.md
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[![Twitter Badge](https://badgen.net/badge/icon/twitter?icon=twitter&label)](https://twitter.com/entracod)


VollSeg is more than just a single segmentation algorithm; it is a meticulously designed modular segmentation tool tailored to diverse model organisms and imaging methods. While a U-Net might suffice for certain image samples, others might benefit from utilizing StarDist, and some could require a blend of both, potentially coupled with denoising or region of interest models. The pivotal decision left to make is how to select the most appropriate VollSeg configuration for your dataset, a question we comprehensively address in our documentation.
VollSeg is more than just a single segmentation algorithm; it is a meticulously designed modular segmentation tool tailored to diverse model organisms and imaging methods. While a U-Net might suffice for certain image samples, others might benefit from utilizing StarDist, and some could require a blend of both, potentially coupled with denoising or region of interest models. The pivotal decision left to make is how to select the most appropriate VollSeg configuration for your dataset, a question we comprehensively address in our [documentation website](https://kapoorlabs-caped.github.io/vollseg-napari/).

This project provides the [napari](https://napari.org/) plugin for [VollSeg](https://github.com/kapoorlab/vollseg), a deep learning based 2D and 3D segmentation tool for irregular shaped cells. VollSeg has originally been developed (see [papers](http://conference.scipy.org/proceedings/scipy2021/varun_kapoor.html)) for the segmentation of densely packed membrane labelled cells in challenging images with low signal-to-noise ratios. The plugin allows to apply pretrained and custom trained models from within napari.
For detailed demo of the plugin see these [videos](https://www.youtube.com/watch?v=W_gKrLWKNpQ) and a short video about the [parameter selection](https://www.youtube.com/watch?v=7tQMn_u8_7s&t=1s)
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4 changes: 2 additions & 2 deletions _build/html/MAMMARYGLAND.html
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Expand Up @@ -358,9 +358,9 @@ <h1>Segmentation of mammary gland cells</h1>

<section class="tex2jax_ignore mathjax_ignore" id="segmentation-of-mammary-gland-cells">
<h1>Segmentation of mammary gland cells<a class="headerlink" href="#segmentation-of-mammary-gland-cells" title="Permalink to this headline">#</a></h1>
<p>VollSeg specializes with its seed pooling approach to segment irregular shapes of</p>
<p>VollSeg specializes with its seed pooling approach to segment irregular shapes of mammary gland cells</p>
<p><img alt="Mammary Gland Cells" src="_images/Seg_compare-big.png" /></p>
<p>from human or mouse samples. In the orignal algorithm we use a CARE trained denoising model and eaither use the U-Net model for semantic segmentation or use the denoising model for the semantic segmentation depending on which model has a better prediction. For using combination of (U-Net, CARE and StarDist) model with U-Net as the model for semantic segmentation use this <a class="reference download internal" download="" href="_downloads/55ced61326261fee22b55dba8817fd8e/mammary_gland_us.py"><span class="xref download myst">script</span></a> if you want to use the denoised image as the base image for creating the semantic segmentation map using Otsu threshold set the parameter <code class="docutils literal notranslate"><span class="pre">dounet=False</span></code> in that same script.</p>
<p>from human or mouse samples. In the orignal algorithm we use a CARE trained denoising model and either use the U-Net model for semantic segmentation or use a denoising model for the semantic segmentation depending on which model has a better prediction. Denoising model is used to denoise the image first and the result is then passed to the segmentation models. For using combination of (U-Net, CARE and StarDist) model with U-Net as the model for semantic segmentation use this <a class="reference download internal" download="" href="_downloads/55ced61326261fee22b55dba8817fd8e/mammary_gland_us.py"><span class="xref download myst">script</span></a> if you want to use the denoised image as the base image for creating the semantic segmentation map using Otsu threshold set the parameter <code class="docutils literal notranslate"><span class="pre">dounet=False</span></code> in that same script.</p>
</section>

<script type="text/x-thebe-config">
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Expand Up @@ -381,7 +381,7 @@ <h2>VollOne Algorithm<a class="headerlink" href="#vollone-algorithm" title="Perm
</section>
<section id="script">
<h2>Script<a class="headerlink" href="#script" title="Permalink to this headline">#</a></h2>
<p>The script used for this step are: <a class="reference download internal" download="" href="_downloads/7ad3a4992554c10f7ec146ec06b4e82c/spheroid_nuclei_membrane_segmentation.py"><span class="xref download myst">script</span></a> and <a class="reference download internal" download="" href="_downloads/9db6ac1e5e9802c20682b7301efe0d04/timelapse_spheroids_joint_segmentation.py"><span class="xref download myst">script</span></a>.</p>
<p>The script used for this step are: (for CZYX) <a class="reference download internal" download="" href="_downloads/7ad3a4992554c10f7ec146ec06b4e82c/spheroid_nuclei_membrane_segmentation.py"><span class="xref download myst">script</span></a> and (for CTZYX) <a class="reference download internal" download="" href="_downloads/9db6ac1e5e9802c20682b7301efe0d04/timelapse_spheroids_joint_segmentation.py"><span class="xref download myst">script</span></a>.</p>
</section>
</section>

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4 changes: 2 additions & 2 deletions _build/html/_sources/MAMMARYGLAND.md
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# Segmentation of mammary gland cells

VollSeg specializes with its seed pooling approach to segment irregular shapes of
VollSeg specializes with its seed pooling approach to segment irregular shapes of mammary gland cells

![Mammary Gland Cells](images/Seg_compare-big.png)

from human or mouse samples. In the orignal algorithm we use a CARE trained denoising model and eaither use the U-Net model for semantic segmentation or use the denoising model for the semantic segmentation depending on which model has a better prediction. For using combination of (U-Net, CARE and StarDist) model with U-Net as the model for semantic segmentation use this [script](scripts/mammary_gland_us.py) if you want to use the denoised image as the base image for creating the semantic segmentation map using Otsu threshold set the parameter ```dounet=False``` in that same script.
from human or mouse samples. In the orignal algorithm we use a CARE trained denoising model and either use the U-Net model for semantic segmentation or use a denoising model for the semantic segmentation depending on which model has a better prediction. Denoising model is used to denoise the image first and the result is then passed to the segmentation models. For using combination of (U-Net, CARE and StarDist) model with U-Net as the model for semantic segmentation use this [script](scripts/mammary_gland_us.py) if you want to use the denoised image as the base image for creating the semantic segmentation map using Otsu threshold set the parameter ```dounet=False``` in that same script.
2 changes: 1 addition & 1 deletion _build/html/_sources/SPHEROIDS.md
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## Script

The script used for this step are: [script](scripts/spheroid_nuclei_membrane_segmentation.py) and [script](scripts/timelapse_spheroids_joint_segmentation.py).
The script used for this step are: (for CZYX) [script](scripts/spheroid_nuclei_membrane_segmentation.py) and (for CTZYX) [script](scripts/timelapse_spheroids_joint_segmentation.py).



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