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# Confocal and Light Sheet imaged Embryonic cells | ||
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## Ascadian Embryo | ||
In this example we consider a dataset imaged using Light sheet fused from four angles to create a single channel 3D image of Phallusia Mammillata Embryo created using live SPIM imaging. The training data can be found [here](https://figshare.com/articles/dataset/Astec-half-Pm1_Cut_at_2-cell_stage_half_Phallusia_mammillata_embryo_live_SPIM_imaging_stages_6-16_/11309570?backTo=/s/765d4361d1b073beedd5). For this imaging modality we trained only a [UNet model](https://zenodo.org/record/6337699) to segment the interior region of the cells and by using ```slice_merge=True``` and | ||
```expand_labels=True``` in the VollSeg parameter setting we obtained the following segmentation result along with the metrics compared to the ground truth. | ||
<img src="https://github.com/kapoorlab/vollseg-napari/blob/main/vollseg_napari/images/Ascadian_raw.png" title="Raw Ascadian Embryo" width="200"> | ||
<img src="https://github.com/kapoorlab/vollseg-napari/blob/main/vollseg_napari/images/Ascadian_pred.png" title="Prediction Ascadian Embryo" width="200" > | <img src="https://github.com/kapoorlab/vollseg-napari/blob/main/vollseg_napari/images/Metrics_Ascadian.png" title="Metrics Ascadian Embryo" width="200" > |
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# Book settings | ||
# Learn more at https://jupyterbook.org/customize/config.html | ||
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title: Segmentation using VollSeg | ||
author: Varun Kapoor | ||
logo: images/kapoorlogo.png | ||
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# Force re-execution of notebooks on each build. | ||
# See https://jupyterbook.org/content/execute.html | ||
execute: | ||
execute_notebooks: force | ||
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# Define the name of the latex output file for PDF builds | ||
latex: | ||
latex_documents: | ||
targetname: vollseg-napari.tex | ||
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# Add a bibtex file so that we can create citations | ||
bibtex_bibfiles: | ||
- references.bib | ||
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# Information about where the book exists on the web | ||
repository: | ||
url: https://github.com/Kapoorlabs-CAPED/vollseg-napari/ # Online location of your book | ||
path_to_book: docs # Optional path to your book, relative to the repository root | ||
branch: master # Which branch of the repository should be used when creating links (optional) | ||
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# Add GitHub buttons to your book | ||
# See https://jupyterbook.org/customize/config.html#add-a-link-to-your-repository | ||
html: | ||
use_issues_button: true | ||
use_repository_button: true |
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# Table of contents | ||
# Learn more at https://jupyterbook.org/customize/toc.html | ||
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format: jb-book | ||
root: README | ||
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chapters: | ||
- file: EMBRYOS | ||
- file: MAMMARYGLAND | ||
- file: XENOPUS | ||
- file: SPHEROIDS | ||
- file: MICROTUBULES |