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[README]: minor
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beyondpie authored Apr 11, 2024
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Expand Up @@ -34,7 +34,18 @@ There are some break changes :https://kzhang.org/SnapATAC2/changelog.html later

[[./repo_figures/snATAC-seq_analysis_pipeline.jpg]]
** Codes
*** [[file:package/R/cembav2env.R][cembav2env.R]]: R env to store the metadata during analysis.
*** Clustering
In total, we have implemented four-round iterative clustering.
See details in [[file:01.clustering][01.clustering]]
*** Integration and annotation
We use Allen's scRNAseq data and their annotations for our data annotation.
See details in [[file:02.integration][02.integration]]
*** Peak calling
We use macs2 with multiple stage filtering, especially use SPM >= 5
for filtering peaks.
See details in [[file:03.peakcalling][03.peakcalling]]
*** Comments on some scripts:
1. [[file:package/R/cembav2env.R][cembav2env.R]]: R env to store the metadata during analysis.
|----------------+-------------------------------------------------------|
| Enviorment | Description |
|----------------+-------------------------------------------------------|
Expand All @@ -47,17 +58,6 @@ There are some break changes :https://kzhang.org/SnapATAC2/changelog.html later
|----------------+-------------------------------------------------------|
| Sa2PeakCalling | Peak calling meta data |
|----------------+-------------------------------------------------------|
*** Clustering
In total, we have implemented four-round iterative clustering.
See details in [[file:01.clustering][01.clustering]]
*** Integration and annotation
We use Allen's scRNAseq data and their annotations for our data annotation.
See details in [[file:02.integration][02.integration]]
*** Peak calling
We use macs2 with multiple stage filtering, especially use SPM >= 5
for filtering peaks.
See details in [[file:03.peakcalling][03.peakcalling]]




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