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updating year
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Luca Cozzuto authored and Luca Cozzuto committed Oct 21, 2024
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4 changes: 2 additions & 2 deletions docs/alignment.md
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Expand Up @@ -39,7 +39,7 @@ cd .. # go up one level to the root of the project, if needed
mkdir alignment
cd alignment
wget https://biocorecrg.github.io/PhD_course_genomics_format_2023/data/H3K4me1.sam.gz
wget https://biocorecrg.github.io/PhD_course_genomics_format_2024/data/H3K4me1.sam.gz
gunzip H3K4me1.sam.gz
more H3K4me1.sam
Expand Down Expand Up @@ -93,7 +93,7 @@ To convert SAM to BAM, sort, index, etc. you can use the program: [**samtools**]


```{bash}
wget https://biocorecrg.github.io/PhD_course_genomics_format_2023/data/H3K4me1.bam
wget https://biocorecrg.github.io/PhD_course_genomics_format_2024/data/H3K4me1.bam
```

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4 changes: 2 additions & 2 deletions docs/fasta_format.md
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Expand Up @@ -51,7 +51,7 @@ cd ../ # to go to the root of the project folder, if needed
mkdir db
cd db
wget https://biocorecrg.github.io/PhD_course_genomics_format_2023/data/Homo_sapiens.GRCh38.dna.chromosome.21.fa.gz
wget https://biocorecrg.github.io/PhD_course_genomics_format_2024/data/Homo_sapiens.GRCh38.dna.chromosome.21.fa.gz
```

The genome is generally represented as a FASTA file (.fa file). Each chromosome sequence starts with a header row, starting with "**>**":
Expand Down Expand Up @@ -182,7 +182,7 @@ The flow of commands is the following:

**EXERCISE**

Download in the folder ./db the annotation file at https://biocorecrg.github.io/PhD_course_genomics_format_2023/data/annotation.gtf.gz
Download in the folder ./db the annotation file at https://biocorecrg.github.io/PhD_course_genomics_format_2024/data/annotation.gtf.gz
* What is the date of this file? (use command **more** to explore the file)
* How many lines in this file?
* How many lines in this file contain the word "HAVANA"?
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10 changes: 5 additions & 5 deletions docs/fastq.md
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Expand Up @@ -47,10 +47,10 @@ It is recommended to always use the date at the beginning of the folder name, in
<br>

```{bash}
mkdir 2023-10-25-genomics_data_formats
mkdir 2024-10-25-genomics_data_formats
# type cd 2[TAB] to autofill the folder name
cd 2023-10-25-genomics_data_formats
cd 2024-10-25-genomics_data_formats
```

<br>
Expand All @@ -61,7 +61,7 @@ Let's use command **cat** to create a simple README.txt

```{bash}
cat > README.txt
Date: 25/10/2023
Date: 25/10/2024
Author: ....
Project title: This is my first project.
```
Expand Down Expand Up @@ -108,7 +108,7 @@ Here my suggestion in case you might want to try **VIM**
Download a **test dataset** by executing the command **wget**:

```{bash}
wget https://biocorecrg.github.io/PhD_course_genomics_format_2023/data/H3K4me1_chr21.fq.gz
wget https://biocorecrg.github.io/PhD_course_genomics_format_2024/data/H3K4me1_chr21.fq.gz
--2019-10-15 14:42:59-- https://biocorecrg.github.io/PhD_course/data/H3K4me1_chr21.fq.gz
Resolving biocorecrg.github.io (biocorecrg.github.io)... 185.199.109.153, 185.199.111.153, 185.199.110.153, ...
Connecting to biocorecrg.github.io (biocorecrg.github.io)|185.199.109.153|:443... connected.
Expand All @@ -134,7 +134,7 @@ ls -lh H3K4me1_chr21.fq.gz
<br/>

**EXERCISE**<br/>
* Download the **control file for this ChIP-seq experiment** at https://biocorecrg.github.io/PhD_course_genomics_format_2023/data/input_chr21.fq.gz <br>
* Download the **control file for this ChIP-seq experiment** at https://biocorecrg.github.io/PhD_course_genomics_format_2024/data/input_chr21.fq.gz <br>
* What is the size of the input file?
* Look up for the options of the command **ls** using the command **man ls**.
* Which option in command **ls** allows to display files sorted by size? Run this command.
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2 changes: 1 addition & 1 deletion docs/genome_browser.md
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Expand Up @@ -47,7 +47,7 @@ You can also display aligned sequences, but you need to place your sorted and in
Copy paste this link into the **custom track**. This is the sorted version of our alignment, hosted in the github web-server.

```{bash}
https://biocorecrg.github.io/PhD_course_genomics_format_2023/data/H3K4me1_s.bam
https://biocorecrg.github.io/PhD_course_genomics_format_2024/data/H3K4me1_s.bam
```

In the same place we also hosted the index, called **H3K4me1_s.bam.bai**. When you don't specify it, the browser will automatically look for the index just adding **.bai** ad the end of the link we provide.
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4 changes: 2 additions & 2 deletions docs/index.md
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Expand Up @@ -4,7 +4,7 @@ title: Home
navigation: 1
---

# ![logo](https://raw.githubusercontent.com/CRG-CNAG/BioCoreMiscOpen/master/logo/biocore-logo_small.png) PhD Course 2023
# ![logo](https://raw.githubusercontent.com/CRG-CNAG/BioCoreMiscOpen/master/logo/biocore-logo_small.png) PhD Course 2024

# Linux commands and Genomics data formats for biologists

Expand All @@ -19,7 +19,7 @@ navigation: 1

from the CRG [Bioinformatics core facility](https://biocore.crg.eu/) (office 425, 4th floor of the hospital side)

Materials for the course are available at [this website](https://biocorecrg.github.io/PhD_course_genomics_format_2023/).
Materials for the course are available at [this website](https://biocorecrg.github.io/PhD_course_genomics_format_2024/).


## Learning objectives
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4 changes: 2 additions & 2 deletions docs/peak_calling.md
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Expand Up @@ -37,7 +37,7 @@ cd ..
mkdir peaks
cd peaks
wget https://biocorecrg.github.io/PhD_course_genomics_format_2023/data/H3K4me_peaks.xls
wget https://biocorecrg.github.io/PhD_course_genomics_format_2024/data/H3K4me_peaks.xls
more H3K4me_peaks.xls
Expand Down Expand Up @@ -81,7 +81,7 @@ chr start end length abs_summit pileup -log10(pvalue) fold_enrichment -log10(qva
Another output file is **H3K4me_peaks.narrowPeak**, which, as you can see contains the information only about peaks. This is a BED file.

```{bash}
wget https://biocorecrg.github.io/PhD_course_genomics_format_2023/data/H3K4me_peaks.narrowPeak
wget https://biocorecrg.github.io/PhD_course_genomics_format_2024/data/H3K4me_peaks.narrowPeak
more H3K4me_peaks.narrowPeak
21 6565480 6565885 H3K4me_peak_1 32 . 3.66432 7.98323 3.26622 153
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4 changes: 2 additions & 2 deletions docs/qc.md
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Expand Up @@ -26,15 +26,15 @@ cd .. # make sure to move up in the root of the project folder
mkdir fastqc
cd fastqc
wget https://biocorecrg.github.io/PhD_course_genomics_format_2023/data/H3K4me1_chr21_fastqc.html
wget https://biocorecrg.github.io/PhD_course_genomics_format_2024/data/H3K4me1_chr21_fastqc.html
firefox H3K4me1_chr21_fastqc.html
```

**NOTE**. If you don't have X11 forwarding, don't worry. Just copy paste this link in your browser:

```{bash}
https://biocorecrg.github.io/PhD_course_genomics_format_2023/data/H3K4me1_chr21_fastqc.html
https://biocorecrg.github.io/PhD_course_genomics_format_2024/data/H3K4me1_chr21_fastqc.html
```

<img src="images/fastqc.png" width="800"/>
Expand Down

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