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2.4.0 Added run-cgprna command line tool and CWL workflows

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@byb121 byb121 released this 08 Oct 12:13
· 61 commits to dev since this release
  • Reduced Docker image size
  • Added run-cgprna command line tool, which can be used to complete a step in RNA-seq data workflow with just one command. Currently 4 subcommands are implemented:
    • map: uses star_mapping.pl to map and marks duplicates after mapping.
    • stats: generates mapping stats using bam_stats and RSeQC.
    • bigwig: generates bigwig file using bamToBw.pl
    • counts: counts reads using htseq-count.
  • Built a new set of reference files for run-cgprna to use. They're available on ftp://ftp.sanger.ac.uk/pub/cancer/support-files/cgpRna_container/.
  • CWL tools and workflows:
    • added a workflow to map sample by lanes, generate mapping stats for lanes, merge lane bams, generate bigwig file and count reads.
    • added CWL tools/workflows for the workflow above to use.
    • added example JSON for most of the CWL files.