dysgu==1.6.4_version update (nf-core#5928)

* all_files_update * Update environment.yml * update_meta.yml * input==input_bam * update_snapshot * meta.yml,tags.yml update * Update modules/nf-core/dysgu/meta.yml Co-authored-by: Famke Bäuerle <[email protected]> * Update modules/nf-core/dysgu/meta.yml Co-authored-by: Famke Bäuerle <[email protected]> * Update modules/nf-core/dysgu/meta.yml Co-authored-by: Famke Bäuerle <[email protected]> * Update modules/nf-core/dysgu/meta.yml Co-authored-by: Famke Bäuerle <[email protected]> * Update modules/nf-core/dysgu/meta.yml Co-authored-by: Famke Bäuerle <[email protected]> * update_author --------- Co-authored-by: Famke Bäuerle <[email protected]>
famosab · Jul 8, 2024 · c274982 · c274982
1 parent bf4f1b9
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diff --git a/modules/nf-core/dysgu/environment.yml b/modules/nf-core/dysgu/environment.yml
@@ -0,0 +1,7 @@
+name: dysgu
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - dysgu
diff --git a/modules/nf-core/dysgu/main.nf b/modules/nf-core/dysgu/main.nf
@@ -0,0 +1,55 @@
+process DYSGU {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'oras://community.wave.seqera.io/library/dysgu:48830f55112c399e':
+        'community.wave.seqera.io/library/dysgu:faf71ac972284412' }"
+
+    input:
+    tuple val(meta), path(input), path(index)
+    tuple val(meta2), path(fasta), path(fai)
+
+    output:
+    tuple val(meta), path('*.vcf.gz')       , emit: vcf
+    tuple val(meta), path('*.vcf.gz.tbi')   , emit: tbi
+    path 'versions.yml'                     , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    def args3 = task.ext.args3 ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    dysgu run \\
+        -p ${task.cpus} \\
+        -x \\
+        $fasta \\
+        . \\
+        $input \\
+        | bgzip ${args2} --threads ${task.cpus} --stdout > ${prefix}.vcf.gz
+    tabix ${args3} ${prefix}.vcf.gz
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        dysgu: \$(dysgu --version 2>&1)
+    END_VERSIONS
+    """
+
+    stub:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    echo "" | gzip > ${prefix}.vcf.gz
+    touch ${prefix}.vcf.gz.tbi
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        dysgu: \$(dysgu --version 2>&1)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/dysgu/meta.yml b/modules/nf-core/dysgu/meta.yml
@@ -0,0 +1,68 @@
+name: dysgu
+
+description: Dysgu calls structural variants (SVs) from mapped sequencing reads. It is designed for accurate and efficient detection of structural variations.
+keywords:
+  - structural variants
+  - sv
+  - vcf
+tools:
+  - dysgu:
+      description: Structural variant caller for mapped sequencing data
+      homepage: https://github.com/kcleal/dysgu
+      documentation: https://github.com/kcleal/dysgu/blob/master/README.rst
+      tool_dev_url: https://github.com/kcleal/dysgu
+      doi: "10.1093/nar/gkac039"
+      licence: ["GPL-3.0-or-later"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. `[ id:'sample1' ]`
+  - input:
+      type: file
+      description: Input BAM file
+      pattern: "*.bam"
+  - index:
+      type: file
+      description: BAM index file
+      pattern: "*.bai"
+  - fasta:
+      type: file
+      description: Genome reference FASTA file
+      pattern: "*.{fa,fasta}"
+  - meta2:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'genome' ]
+  - fai:
+      type: file
+      description: Genome reference FASTA index file
+      pattern: "*.{fa.fai,fasta.fai}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'sample1' ]
+  - vcf:
+      type: file
+      description: VCF file with identified structural variants
+      pattern: "*.{vcf.gz}"
+  - tbi:
+      type: file
+      description: The index of the BCF/VCF file
+      pattern: "*.{vcf.gz.tbi}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@famosab"
+  - "@poddarharsh15"
+maintainers:
+  - "@poddarharsh15"
diff --git a/modules/nf-core/dysgu/tests/main.nf.test b/modules/nf-core/dysgu/tests/main.nf.test
@@ -0,0 +1,96 @@
+nextflow_process {
+
+    name "Test Process DYSGU"
+    script "../main.nf"
+    process "DYSGU"
+    config "./nextflow.config"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "dysgu"
+
+
+    test("human - bam") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test'], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.recalibrated.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.recalibrated.sorted.bam.bai', checkIfExists: true)
+                ]
+                input[1] = [ [ id:'reference'], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.versions).match() },
+                { assert path(process.out.vcf.get(0).get(1)).linesGzip.contains("##fileformat=VCFv4.2") }
+            )
+        }
+
+    }
+
+
+    test("human - cram") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test'], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram.crai', checkIfExists: true)
+                ]
+                input[1] = [ [ id:'reference'], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.versions).match() },
+                { assert path(process.out.vcf.get(0).get(1)).linesGzip.contains("##fileformat=VCFv4.2") }
+            )
+        }
+
+    }
+
+
+    test("human - bam - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test'], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.recalibrated.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.recalibrated.sorted.bam.bai', checkIfExists: true)
+                ]
+                input[1] = [ [ id:'reference'], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/dysgu/tests/main.nf.test.snap b/modules/nf-core/dysgu/tests/main.nf.test.snap
@@ -0,0 +1,75 @@
+{
+    "human - bam - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,cf1e0487502108690603dd16f034bf5e"
+                ],
+                "tbi": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,cf1e0487502108690603dd16f034bf5e"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-08T13:18:45.660262"
+    },
+    "human - bam": {
+        "content": [
+            [
+                "versions.yml:md5,cf1e0487502108690603dd16f034bf5e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-08T13:19:56.62312"
+    },
+    "human - cram": {
+        "content": [
+            [
+                "versions.yml:md5,cf1e0487502108690603dd16f034bf5e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-08T13:20:04.494134"
+    }
+}
diff --git a/modules/nf-core/dysgu/tests/nextflow.config b/modules/nf-core/dysgu/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: DYSGU {
+         ext.args = '--exome '
+    }
+}
diff --git a/modules/nf-core/dysgu/tests/tags.yml b/modules/nf-core/dysgu/tests/tags.yml
@@ -0,0 +1,2 @@
+dysgu:
+  - "modules/nf-core/dysgu/**"