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Add the option to change the default starting contig index kmer size.…
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… Update the example output results.
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@mourisl
mourisl committed Dec 9, 2022
1 parent 79f2cf6 commit 36ffe8a
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1 change: 1 addition & 0 deletions README.md
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Expand Up @@ -40,6 +40,7 @@ TRUST4 is also available form [Bioconda](https://anaconda.org/bioconda/trust4).
-o STRING: prefix of output files. (default: inferred from file prefix)
--od STRING: the directory for output files. (default: ./)
-t INT: number of threads (default: 1)
-k INT: the starting k-mer size for indexing contigs (default: 9)
--barcode STRING: if -b, bam field for barcode; if -1 -2/-u, file containing barcodes (defaul: not used)
--barcodeRange INT INT CHAR: start, end(-1 for lenght-1), strand in a barcode is the true barcode (default: 0 -1 +)
--barcodeWhitelist STRING: path to the barcode whitelist (default: not used)
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12 changes: 6 additions & 6 deletions example/TRUST_example_airr.tsv
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12 changes: 6 additions & 6 deletions example/TRUST_example_cdr3.out
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assemble0 0 IGHV5-51*01 * * * GGATACAGCTTTACCAGCTACTGG ATCTATCCTGGTGACTCTGATACC TGTGCGAGACATCTTGGAGATTGTGGTGGTGACTGC 0.00 3.00 0.00 0
assemble1 0 IGHV1-3*01 IGHD1-26*01 IGHJ6*04 * GGATACACCTTCACTAGCTATGCT ATCAACGCTGGCAATGGTAACACA TGTGCGAGAGGGCAGGACGGTGACTACGTGGGCGAGCCCGGGGAGTCTACTACTACTACTACATGGACGTCTGG 1.00 4.00 94.00 0
assemble2 0 IGHV4-61*01 IGHD2-2*01 IGHJ3*02 * GGTGGCTCCGTCAGCAGTGGTAGTTACTAC ATCTATTACAGTGGGAGCACC TGTGCGAGGGGGCGAACGATAATATTGTAGTAGTACCAGCTGCTATCTTCCATTTGCTTTTGATATCTGG 1.00 3.00 100.00 0
assemble3 0 IGHV3-NL1*01 IGHD2-21*02 IGHJ6*02 * GGATTCACCTTCAGTAGCTATGGC ATTTATAGCGGTGGTAGTAGCACA TGTGCGAAGGAGGTTGGGTGGTGACTGCTAGGGAAACTACTACTACGGTATGGACGTCTGG 1.00 2.00 93.88 0
assemble1 0 IGHV1-3*01 IGHD1-26*01 IGHJ6*04 * GGATACACCTTCACTAGCTATGCT ATCAACGCTGGCAATGGTAACACA TGTGCGAGAGGGCAGGACGGTGACTACGTGGGCGAGCCCGGGGAGTCTACTACTACTACTACATGGACGTCTGG 1.00 8.00 94.00 0
assemble2 0 IGHV4-61*01 IGHD2-2*01 IGHJ3*02 * GGTGGCTCCGTCAGCAGTGGTAGTTACTAC ATCTATTACAGTGGGAGCACC TGTGCGAGGGGGCGAACGATAATATTGTAGTAGTACCAGCTGCTATCTTCCATTTGCTTTTGATATCTGG 1.00 6.00 100.00 0
assemble3 0 IGHV3-NL1*01 IGHD2-21*02 IGHJ6*02 * GGATTCACCTTCAGTAGCTATGGC ATTTATAGCGGTGGTAGTAGCACA TGTGCGAAGGAGGTTGGGTGGTGACTGCTAGGGAAACTACTACTACGGTATGGACGTCTGG 1.00 3.00 93.88 0
assemble4 0 IGHV3-7*03 * * * GGATTCACCTTCAGTAGCTATTGC ATAAAGCAAGATGGAAGTGAGAAA TGTGCGAGAGGGAGATATTGT 0.00 1.00 0.00 0
assemble5 0 IGHV3-11*01 IGHD6-19*01 IGHJ4*02 IGHM GGATTCACCTTCAGTGACTACTAC ATTAGTAGTAGTGGTAGTACCATA TGTGCGAGAGACCCTCCCCGGAGCAGTGGCTGGTGGGCGTCCCCTCTTCGTTTTTGACTACTGG 1.00 3.00 100.00 0
assemble6 0 IGHV3-23*01 * IGHJ4*02 IGHM GGATTCACCTTTAGCAGCTATGCC ATTAGTGGTAGTGGTGGTAGCACA GCGAAAGCCGCATGACTCACGTACAGTAACTCCACATTTGACTACTGG 0.00 1.00 0.00 0
assemble7 0 IGHV1-2*04 IGHD4-17*01 IGHJ4*02 IGHM GGATACACCTTCACCGGCTACTAT ATCAACCCTAACAGTGGTGGCACA TGTGCGAGAGACTTAGCAGTGGCTCCCCCGGCTAGTACTACTTTGACTACTGG 1.00 1.00 95.00 0
assemble9 0 IGHV1-69*01 IGHD2-8*02 IGHJ6*04 IGHM GGAGGCACCTTCAGCAGCTATGCT ATCATCCTTATCTTTGGTACAGCA TGTGCGAGCAACGCTGGATTGTACTGGTGGTGTATGCTAAACAATGGATTTAGATCGAGGGGAAGAAGTTACTACTACTACTACATGGACGTCTGG 1.00 2.00 93.55 0
assemble10 0 IGHV3-48*04 IGHD3-3*01 IGHJ6*02 IGHM GGATTCACCTTCAGTAGCTATTGC ATTAGTAGTAGTAGTACCATA GGGGTATTACGATTTTTGGAGTGGTTATAAAAGCGGGGGTACTACTACTACGGTATGGACGTCTGG 0.83 3.00 0.00 0
assemble10 0 IGHV3-48*04 IGHD3-3*01 IGHJ6*02 IGHM GGATTCACCTTCAGTAGCTATTGC ATTAGTAGTAGTAGTACCATA GGGGTATTACGATTTTTGGAGTGGTTATAAAAGCGGGGGTACTACTACTACGGTATGGACGTCTGG 0.83 4.00 0.00 0
assemble11 0 IGHV5-51*01 IGHD1-26*01 IGHJ3*02 IGHM * ATCTATCCTGGTGACTCTGATACC TGTGCGAGACAAGTGGGAGCGACCCTCATGATGCTTTTGATATCTGG 1.00 3.00 97.50 0
assemble12 0 IGHV3-69-1*01 IGHD2-15*01 IGHJ5*02 * * ATTAGTAGTAGTAGTACCATA TGTGCGAGAAAACCGACCCGGAAACGGATATTGTAGTGGTGGTAGCTGCTACTCGCGGTACCCTCTACACTTGGTTCGACCCCTGG 1.00 3.00 100.00 0
assemble13 0 IGHV1-18*01 IGHD2-15*01 IGHJ6*04 IGHM * * TGTGCGAGAAATGGGAGTTCGGATTTTGATCCAATCTCCTCGGGGGATTGTAGTAGTACCAGCTGCCTACTACTACTACTACATGGACGTCTGG 1.00 3.00 88.52 0
assemble15 0 IGHV3-47*01 IGHD2-2*02 IGHJ4*02 IGHM * ATTGGTACTGGTGGTGATACA TGTGCAAGAGACCTAAGACATACCAGCTGCTATACTTTGACTACTGG 1.00 2.00 100.00 0
assemble16 0 * * IGHJ6*02 IGHM * * GGGTATAGCAGTGGCTGGTCCACGTACTACTACGGTATGGACGTCTGG 0.00 1.00 0.00 0
assemble17 0 IGHV3-71*01 IGHD6-19*01 IGHJ5*02 * * ATTAGAAACAAAGCTAATGGTGGGACAACA TGTGCGAGAGACCTCGGCCGTAGGTATAGCAGTGGCTGGTTCTGTTTCGACCCCTGG 1.00 1.00 100.00 0
assemble19 0 IGHV3-13*01 IGHD2-2*01 IGHJ6*02 * * ATTGGTACTGCTGGTGACACA TGTTATTGTAGTAGTACCAGCTGCTATTACTACTACTACGGTATGGACGTCTGG 1.00 2.00 94.74 0
assemble21 0 IGHV4-61*01,IGHV4-59*01 IGHD2-2*01 IGHJ4*02 IGHM * ATCTATTACAGTGGGAGCACC TGTGCGAGAGATGGTACCCCGGATGTAGTAGTACCAGCTGCTATGTTTCTTAAACCGGTTGTCTGACTACTGG 1.00 3.00 100.00 0
assemble21 0 IGHV4-61*01,IGHV4-59*01 IGHD2-2*01 IGHJ4*02 IGHM * ATCTATTACAGTGGGAGCACC TGTGCGAGAGATGGTACCCCGGATGTAGTAGTACCAGCTGCTATGTTTCTTAAACCGGTTGTCTGACTACTGG 1.00 5.00 100.00 0
assemble22 0 IGHV3-74*01 * * * GGATTCACCTTCAGTAGCTACTGG ATTAATAGTGATGGGAGTAGCACA TGTGCAAGCATTTGAATATAGCAGCTCGTCATTGTGATAACT 0.00 2.00 0.00 0
assemble24 0 IGHV6-1*01 * * * GGGGACAGTGTCTCTAGCAACAGTGCTGCT ACATACTACAGGTCCAAGTGGTATAAT TGTGCAAGACCTTCCGCATAGCAGTGG 0.00 1.00 0.00 0
assemble25 0 IGHV3-74*01 IGHD2-15*01 IGHJ5*02 IGHM * ATTAATAGTGATGGGAGTAGCACA TGTGCAAGAGATGTAGGAGGGGTATTTCCATTGTAGTGGTGGTAGCTGCTATGCTGGTTCGACCCCTGG 1.00 3.00 100.00 0
Expand All @@ -28,7 +28,7 @@ assemble31 0 IGHV3-23*01 IGHD2-2*01 IGHJ4*02 IGHM * * TGTGCGAAAGACGTTGCAAGCACCAG
assemble32 0 IGHV1-3*01 * * * * ATCAACGCTGGCAATGGTAACACA TGTGCGAGAGTAAGAGCCGCTGTCTATTGTAGTAGTACCAGCTGG 0.00 2.00 0.00 0
assemble33 0 IGHV3-41*02 IGHD3-10*01 IGHJ3*02 IGHM * * TGTGCGGCTTTACTATGGTTCAGCCCGGGGTGCTTTTGATATCTGG 1.00 2.00 100.00 0
assemble34 0 IGHV3-66*01 IGHD2-15*01 IGHJ4*02 IGHM * * TGTGCGAGAGATATTGTTGTAGTGGTGGTAGCTGCTACTCCCAGGGCCCAAATTTACTGG 1.00 2.00 100.00 0
assemble36 0 IGHV3-NL1*01 IGHD6-6*01 IGHJ6*02 * * ATTTATAGCGGTGGTAGTAGCACA TGTGCGAAAGATCTAGGAAAGGTAGTCGCCGTATAGCAGCTCGTCCGGAGGGGATTTCTTCCTACTACTACTACGGTATGGACGTCTGG 1.00 2.00 94.55 0
assemble36 0 IGHV3-NL1*01 IGHD6-6*01 IGHJ6*02 * * ATTTATAGCGGTGGTAGTAGCACA TGTGCGAAAGATCTAGGAAAGGTAGTCGCCGTATAGCAGCTCGTCCGGAGGGGATTTCTTCCTACTACTACTACGGTATGGACGTCTGG 1.00 3.00 94.55 0
assemble37 0 IGHV1-3*01 IGHD6-13*01 IGHJ6*02 IGHM * * TGTGCGAGACCCTATAGCAGCAGCTGGTACGAAAGTTTACTACTACTACTACGGTATGGACGTCTGG 1.00 3.00 100.00 0
assemble38 0 IGHV4-59*10 * * * GGTGGCTCCATCAGTAGTTACTAC ATCTATTACAGTGGGAGCACC TGTGCGAGGGGGCGAACG 0.00 1.00 0.00 0
assemble39 0 IGHV1-3*01 IGHD1-1*01 IGHJ5*02 * GGATACACCTTCACTAGCTATGCT ATCAACGCTGGCAATGGTAACACA TGTGCGATGGGGTGGGTCGGTCCTCGGGCAGTACAACTGGTTCGACCCCTGG 1.00 2.00 95.12 0
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86 changes: 43 additions & 43 deletions example/TRUST_example_report.tsv
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#count frequency CDR3nt CDR3aa V D J C cid cid_full_length
4 4.761905e-02 TGTGCGAGAGGGCAGGACGGTGACTACGTGGGCGAGCCCGGGGAGTCTACTACTACTACTACATGGACGTCTGG out_of_frame IGHV1-3*01 IGHD1-26*01 IGHJ6*04 . assemble1 0
3 3.571429e-02 TGTGCGAGACCCTATAGCAGCAGCTGGTACGAAAGTTTACTACTACTACTACGGTATGGACGTCTGG out_of_frame IGHV1-3*01 IGHD6-13*01 IGHJ6*02 IGHM assemble37 0
3 3.571429e-02 TGTGCGAGAAAACCGACCCGGAAACGGATATTGTAGTGGTGGTAGCTGCTACTCGCGGTACCCTCTACACTTGGTTCGACCCCTGG out_of_frame IGHV3-69-1*01 IGHD2-15*01 IGHJ5*02 . assemble12 0
3 3.571429e-02 TGTGCGAGAGACCCTCCCCGGAGCAGTGGCTGGTGGGCGTCCCCTCTTCGTTTTTGACTACTGG out_of_frame IGHV3-11*01 IGHD6-19*01 IGHJ4*02 IGHM assemble5 0
3 3.571429e-02 TGTGCGAGACAAGTGGGAGCGACCCTCATGATGCTTTTGATATCTGG out_of_frame IGHV5-51*01 IGHD1-26*01 IGHJ3*02 IGHM assemble11 0
3 3.571429e-02 TGTGCGAGGGGGCGAACGATAATATTGTAGTAGTACCAGCTGCTATCTTCCATTTGCTTTTGATATCTGG out_of_frame IGHV4-61*01 IGHD2-2*01 IGHJ3*02 . assemble2 0
3 3.571429e-02 GGGGTATTACGATTTTTGGAGTGGTTATAAAAGCGGGGGTACTACTACTACGGTATGGACGTCTGG GVLRFLEWL_KRGYYYYGMDVW IGHV3-48*04 IGHD3-3*01 IGHJ6*02 IGHM assemble10 0
3 3.571429e-02 TGTGCGAGAGATGGTACCCCGGATGTAGTAGTACCAGCTGCTATGTTTCTTAAACCGGTTGTCTGACTACTGG out_of_frame IGHV4-61*01 IGHD2-2*01 IGHJ4*02 IGHM assemble21 0
3 3.571429e-02 TGTGCGAGCGCAGCACGGGATATTGTAGTGGTGGTAGCTGCTACGGTTCATTTATGACTACTGG out_of_frame IGHV3-11*04 IGHD2-15*01 IGHJ4*02 IGHM assemble26 0
3 3.571429e-02 TGTGCGAGAAATGGGAGTTCGGATTTTGATCCAATCTCCTCGGGGGATTGTAGTAGTACCAGCTGCCTACTACTACTACTACATGGACGTCTGG out_of_frame IGHV1-18*01 IGHD2-15*01 IGHJ6*04 IGHM assemble13 0
3 3.571429e-02 TGTGCAAGAGATGTAGGAGGGGTATTTCCATTGTAGTGGTGGTAGCTGCTATGCTGGTTCGACCCCTGG CARDVGGVFPL_WW_LLCWFDPW IGHV3-74*01 IGHD2-15*01 IGHJ5*02 IGHM assemble25 0
2 2.380952e-02 TGTGCGAGAGAGGGATAGCAGTGGCTTGGGTACTACTACTACTACATGGACGTCTGG CAREG_QWLGYYYYYMDVW IGHV5-51*01 IGHD6-19*01 IGHJ6*03 . assemble40 0
2 2.380952e-02 TGTGTGTCCCTCAGAATTACTATGGTTCAGATTCGAACTCGAAGAACTACTACTACTACTACATGGACGTCTGG out_of_frame IGHV1-NL1*01 IGHD3-10*01 IGHJ6*04 IGHM assemble28 0
2 2.380952e-02 TGTGGGGTTCGGGGAGTTAACATCGATAAGAAGTAATACTACTACTACTACGGTATGGACGTCTGG CGVRGVNIDKK_YYYYYGMDVW IGHV3-49*02 IGHD3-10*01 IGHJ6*02 IGHM assemble61 0
2 2.380952e-02 TGTGCGAGAGTGCAAGTGACCTAATAGCAGTGGCTGGTACGATTCCATACTTTGACTACTGG out_of_frame IGHV1-46*01 IGHD6-19*01 IGHJ4*02 IGHM assemble64 0
2 2.380952e-02 TGTGCGGCTTTACTATGGTTCAGCCCGGGGTGCTTTTGATATCTGG out_of_frame IGHV3-41*02 IGHD3-10*01 IGHJ3*02 IGHM assemble33 0
2 2.380952e-02 TGTGCGAGGGTACCCCGCGATGATAGTAGTGGTTAAACAACCCTTTGACTACTGG out_of_frame IGHV4-38-2*01 IGHD3-22*01 IGHJ4*02 IGHM assemble27 0
2 2.380952e-02 TGTGCGAAGGAGGTTGGGTGGTGACTGCTAGGGAAACTACTACTACGGTATGGACGTCTGG out_of_frame IGHV3-NL1*01 IGHD2-21*02 IGHJ6*02 . assemble3 0
2 2.380952e-02 TGTGCGAGCAACGCTGGATTGTACTGGTGGTGTATGCTAAACAATGGATTTAGATCGAGGGGAAGAAGTTACTACTACTACTACATGGACGTCTGG CASNAGLYWWCMLNNGFRSRGRSYYYYYMDVW IGHV1-69*01 IGHD2-8*02 IGHJ6*04 IGHM assemble9 0
2 2.380952e-02 TGTGCACGGCACCCGGGTCTGTGCCTAGTATTACTATGGTTCAGGGATACTACTACTACATGGACGTCTGG out_of_frame IGHV2-70*01 IGHD3-10*01 IGHJ6*04 IGHM assemble44 0
2 2.380952e-02 TGTGCGAAAGATCTAGGAAAGGTAGTCGCCGTATAGCAGCTCGTCCGGAGGGGATTTCTTCCTACTACTACTACGGTATGGACGTCTGG out_of_frame IGHV3-NL1*01 IGHD6-6*01 IGHJ6*02 . assemble36 0
2 2.380952e-02 TGTGCGAAAGACGTTGCAAGCACCAGGGCACTGCCGAAACCCCCAGTTGTAGTAGTACCAGCTGCTATCCCCTTTGACTACTGG CAKDVASTRALPKPPVVVVPAAIPFDYW IGHV3-23*01 IGHD2-2*01 IGHJ4*02 IGHM assemble31 0
2 2.380952e-02 TGTTATTGTAGTAGTACCAGCTGCTATTACTACTACTACGGTATGGACGTCTGG CYCSSTSCYYYYYGMDVW IGHV3-13*01 IGHD2-2*01 IGHJ6*02 . assemble19 0
2 2.380952e-02 TGTAGTGGTGGTAGCTGCTATAGTAAGTACTACTTTGACGACTGG CSGGSCYSKYYFDDW IGHV3-23*01 IGHD2-15*01 IGHJ4*02 IGHM assemble46 0
2 2.380952e-02 TGTGCAAGAGACCTAAGACATACCAGCTGCTATACTTTGACTACTGG out_of_frame IGHV3-47*01 IGHD2-2*02 IGHJ4*02 IGHM assemble15 0
2 2.380952e-02 TGTGCGAGAGATAGGGAGTTGTAGTAGTACCAGCTGCTGTCACGGGACTACTACTACTACTACTACATGGACGTCTGG CARDREL__YQLLSRDYYYYYYMDVW IGHV3-11*04 IGHD2-2*01 IGHJ6*04 IGHM assemble30 0
2 2.380952e-02 TGTGCGAGAGATATTGTTGTAGTGGTGGTAGCTGCTACTCCCAGGGCCCAAATTTACTGG CARDIVVVVVAATPRAQIYW IGHV3-66*01 IGHD2-15*01 IGHJ4*02 IGHM assemble34 0
2 2.380952e-02 TGTGCGATGGGGTGGGTCGGTCCTCGGGCAGTACAACTGGTTCGACCCCTGG out_of_frame IGHV1-3*01 IGHD1-1*01 IGHJ5*02 . assemble39 0
2 2.380952e-02 TGTGCGAGAGGCGATACCCGGTATTACGATTTTTGGAGTGGTTATTATCCGCAAAGAGGACCAGGGCAGTCCTACTACTACTACGGTATGGACGTCTGG CARGDTRYYDFWSGYYPQRGPGQSYYYYGMDVW IGHV3-11*04 IGHD3-3*01 IGHJ6*02 IGHM assemble41 0
1 1.190476e-02 TGTGTGAGAGGGGCCATCCCCTACGGTGAGTAAACACGAATATTACTACTACTACTACATGGACGTCTGG out_of_frame IGHV3-52*01 IGHD4-23*01 IGHJ6*04 IGHM assemble60 0
1 1.190476e-02 TGTGCGAGAGACCCCTATTACTATGATAGTAGTGGTTATATGCCGTGACCCAGCTCGACCCCTGG out_of_frame IGHV4-61*01 IGHD3-22*01 IGHJ5*02 . assemble79 0
1 1.190476e-02 TGTGCGAGGACCGGCCCTATAGCAGTGGCTGGTTTCACTAGGTTACTGG out_of_frame IGHV1-46*01 IGHD6-19*01 IGHJ4*02 . assemble77 0
1 1.190476e-02 TGTGCGAGAGATGAGATTACGATATTTTGACTGGTTATTATCTGACCGAGCTGGTTTTGAGTTCCCTGAAATCCACCTAACCTGCGCAACTACTGG CARDEITIF_LVII_PSWF_VP_NPPNLRNYW IGHV1-3*01 IGHD3-9*01 IGHJ4*02 . assemble57 0
1 1.190476e-02 TGTGCGAACGGAGGCAGCAGAAAGGTGTCTTGGGATGGTTCGACCCCTGG out_of_frame IGHV3-NL1*01 IGHD3-10*01 IGHJ5*02 . assemble53 0
1 1.190476e-02 TGTGCGAGAGACTTAGCAGTGGCTCCCCCGGCTAGTACTACTTTGACTACTGG out_of_frame IGHV1-2*04 IGHD4-17*01 IGHJ4*02 IGHM assemble7 0
1 1.190476e-02 TGTGCGAGAGATGGAAGCGGCGTATTACGATTTTTGGAGTGGTTCTTCCGACCATACTACTACTACTACATGGACGTCTGG CARDGSGVLRFLEWFFRPYYYYYMDVW IGHV3-11*04 IGHD3-3*01 IGHJ6*04 IGHM assemble67 0
1 1.190476e-02 TGTGCGAGAGACCTCGGCCGTAGGTATAGCAGTGGCTGGTTCTGTTTCGACCCCTGG CARDLGRRYSSGWFCFDPW IGHV3-71*01 IGHD6-19*01 IGHJ5*02 . assemble17 0
1 1.190476e-02 TGTGCGAAAGATATACTAGTATGATAGTAGTGGTTATGGGTTCATTGTTTACTACTGG out_of_frame IGHV3-NL1*01 IGHD3-22*01 IGHJ4*02 IGHM assemble75 0
1 1.190476e-02 TGTGCGAGAGCTGTAACTTATATTGTAGTAGTACCAGCTGCGGGCTGACAGTTTACTACTACTACTACGGTATGGACGTCTGG out_of_frame IGHV1-3*01 IGHD2-2*01 IGHJ6*02 IGHM assemble50 0
1 1.190476e-02 TGTGCGAAAGATAGTAGTGGCTACGATTGAATGTTGACTACTGG out_of_frame IGHV3-NL1*01 IGHD5-12*01 IGHJ4*02 . assemble58 0
1 1.190476e-02 TGTGCGAGAGACCGGCGTTCGTGATACAGCTATGGTTACCGGACACTCCTACTACTACATGGACGTCTGG out_of_frame IGHV1-18*01 IGHD5-18*01 IGHJ6*04 IGHM assemble49 0
1 1.190476e-02 TGTGCGAGAGATCGGAGATACCCCTTGACAAGGTATAGCAGTGGCTGGTACGAAATTAATGATGCTTTTGATATCTGG CARDRRYPLTRYSSGWYEINDAFDIW IGHV3-11*01 IGHD6-19*01 IGHJ3*02 . assemble69 0
1 1.190476e-02 TGTGCGAAAGATCTCCCGAACTTATGTATATGTCTGGTGGTTCGACCCCTGG out_of_frame IGHV3-NL1*01 IGHD2-8*02 IGHJ5*02 IGHM assemble56 0
8 8.333333e-02 TGTGCGAGAGGGCAGGACGGTGACTACGTGGGCGAGCCCGGGGAGTCTACTACTACTACTACATGGACGTCTGG out_of_frame IGHV1-3*01 IGHD1-26*01 IGHJ6*04 . assemble1 0
6 6.250000e-02 TGTGCGAGGGGGCGAACGATAATATTGTAGTAGTACCAGCTGCTATCTTCCATTTGCTTTTGATATCTGG out_of_frame IGHV4-61*01 IGHD2-2*01 IGHJ3*02 . assemble2 0
5 5.208333e-02 TGTGCGAGAGATGGTACCCCGGATGTAGTAGTACCAGCTGCTATGTTTCTTAAACCGGTTGTCTGACTACTGG out_of_frame IGHV4-61*01 IGHD2-2*01 IGHJ4*02 IGHM assemble21 0
4 4.166667e-02 GGGGTATTACGATTTTTGGAGTGGTTATAAAAGCGGGGGTACTACTACTACGGTATGGACGTCTGG GVLRFLEWL_KRGYYYYGMDVW IGHV3-48*04 IGHD3-3*01 IGHJ6*02 IGHM assemble10 0
3 3.125000e-02 TGTGCGAGACCCTATAGCAGCAGCTGGTACGAAAGTTTACTACTACTACTACGGTATGGACGTCTGG out_of_frame IGHV1-3*01 IGHD6-13*01 IGHJ6*02 IGHM assemble37 0
3 3.125000e-02 TGTGCGAGAAAACCGACCCGGAAACGGATATTGTAGTGGTGGTAGCTGCTACTCGCGGTACCCTCTACACTTGGTTCGACCCCTGG out_of_frame IGHV3-69-1*01 IGHD2-15*01 IGHJ5*02 . assemble12 0
3 3.125000e-02 TGTGCGAGAGACCCTCCCCGGAGCAGTGGCTGGTGGGCGTCCCCTCTTCGTTTTTGACTACTGG out_of_frame IGHV3-11*01 IGHD6-19*01 IGHJ4*02 IGHM assemble5 0
3 3.125000e-02 TGTGCGAGACAAGTGGGAGCGACCCTCATGATGCTTTTGATATCTGG out_of_frame IGHV5-51*01 IGHD1-26*01 IGHJ3*02 IGHM assemble11 0
3 3.125000e-02 TGTGCGAAGGAGGTTGGGTGGTGACTGCTAGGGAAACTACTACTACGGTATGGACGTCTGG out_of_frame IGHV3-NL1*01 IGHD2-21*02 IGHJ6*02 . assemble3 0
3 3.125000e-02 TGTGCGAGCGCAGCACGGGATATTGTAGTGGTGGTAGCTGCTACGGTTCATTTATGACTACTGG out_of_frame IGHV3-11*04 IGHD2-15*01 IGHJ4*02 IGHM assemble26 0
3 3.125000e-02 TGTGCGAAAGATCTAGGAAAGGTAGTCGCCGTATAGCAGCTCGTCCGGAGGGGATTTCTTCCTACTACTACTACGGTATGGACGTCTGG out_of_frame IGHV3-NL1*01 IGHD6-6*01 IGHJ6*02 . assemble36 0
3 3.125000e-02 TGTGCGAGAAATGGGAGTTCGGATTTTGATCCAATCTCCTCGGGGGATTGTAGTAGTACCAGCTGCCTACTACTACTACTACATGGACGTCTGG out_of_frame IGHV1-18*01 IGHD2-15*01 IGHJ6*04 IGHM assemble13 0
3 3.125000e-02 TGTGCAAGAGATGTAGGAGGGGTATTTCCATTGTAGTGGTGGTAGCTGCTATGCTGGTTCGACCCCTGG CARDVGGVFPL_WW_LLCWFDPW IGHV3-74*01 IGHD2-15*01 IGHJ5*02 IGHM assemble25 0
2 2.083333e-02 TGTGCGAGAGAGGGATAGCAGTGGCTTGGGTACTACTACTACTACATGGACGTCTGG CAREG_QWLGYYYYYMDVW IGHV5-51*01 IGHD6-19*01 IGHJ6*03 . assemble40 0
2 2.083333e-02 TGTGTGTCCCTCAGAATTACTATGGTTCAGATTCGAACTCGAAGAACTACTACTACTACTACATGGACGTCTGG out_of_frame IGHV1-NL1*01 IGHD3-10*01 IGHJ6*04 IGHM assemble28 0
2 2.083333e-02 TGTGGGGTTCGGGGAGTTAACATCGATAAGAAGTAATACTACTACTACTACGGTATGGACGTCTGG CGVRGVNIDKK_YYYYYGMDVW IGHV3-49*02 IGHD3-10*01 IGHJ6*02 IGHM assemble61 0
2 2.083333e-02 TGTGCGAGAGTGCAAGTGACCTAATAGCAGTGGCTGGTACGATTCCATACTTTGACTACTGG out_of_frame IGHV1-46*01 IGHD6-19*01 IGHJ4*02 IGHM assemble64 0
2 2.083333e-02 TGTGCGGCTTTACTATGGTTCAGCCCGGGGTGCTTTTGATATCTGG out_of_frame IGHV3-41*02 IGHD3-10*01 IGHJ3*02 IGHM assemble33 0
2 2.083333e-02 TGTGCGAGGGTACCCCGCGATGATAGTAGTGGTTAAACAACCCTTTGACTACTGG out_of_frame IGHV4-38-2*01 IGHD3-22*01 IGHJ4*02 IGHM assemble27 0
2 2.083333e-02 TGTGCGAGCAACGCTGGATTGTACTGGTGGTGTATGCTAAACAATGGATTTAGATCGAGGGGAAGAAGTTACTACTACTACTACATGGACGTCTGG CASNAGLYWWCMLNNGFRSRGRSYYYYYMDVW IGHV1-69*01 IGHD2-8*02 IGHJ6*04 IGHM assemble9 0
2 2.083333e-02 TGTGCACGGCACCCGGGTCTGTGCCTAGTATTACTATGGTTCAGGGATACTACTACTACATGGACGTCTGG out_of_frame IGHV2-70*01 IGHD3-10*01 IGHJ6*04 IGHM assemble44 0
2 2.083333e-02 TGTGCGAAAGACGTTGCAAGCACCAGGGCACTGCCGAAACCCCCAGTTGTAGTAGTACCAGCTGCTATCCCCTTTGACTACTGG CAKDVASTRALPKPPVVVVPAAIPFDYW IGHV3-23*01 IGHD2-2*01 IGHJ4*02 IGHM assemble31 0
2 2.083333e-02 TGTTATTGTAGTAGTACCAGCTGCTATTACTACTACTACGGTATGGACGTCTGG CYCSSTSCYYYYYGMDVW IGHV3-13*01 IGHD2-2*01 IGHJ6*02 . assemble19 0
2 2.083333e-02 TGTAGTGGTGGTAGCTGCTATAGTAAGTACTACTTTGACGACTGG CSGGSCYSKYYFDDW IGHV3-23*01 IGHD2-15*01 IGHJ4*02 IGHM assemble46 0
2 2.083333e-02 TGTGCAAGAGACCTAAGACATACCAGCTGCTATACTTTGACTACTGG out_of_frame IGHV3-47*01 IGHD2-2*02 IGHJ4*02 IGHM assemble15 0
2 2.083333e-02 TGTGCGAGAGATAGGGAGTTGTAGTAGTACCAGCTGCTGTCACGGGACTACTACTACTACTACTACATGGACGTCTGG CARDREL__YQLLSRDYYYYYYMDVW IGHV3-11*04 IGHD2-2*01 IGHJ6*04 IGHM assemble30 0
2 2.083333e-02 TGTGCGAGAGATATTGTTGTAGTGGTGGTAGCTGCTACTCCCAGGGCCCAAATTTACTGG CARDIVVVVVAATPRAQIYW IGHV3-66*01 IGHD2-15*01 IGHJ4*02 IGHM assemble34 0
2 2.083333e-02 TGTGCGATGGGGTGGGTCGGTCCTCGGGCAGTACAACTGGTTCGACCCCTGG out_of_frame IGHV1-3*01 IGHD1-1*01 IGHJ5*02 . assemble39 0
2 2.083333e-02 TGTGCGAGAGGCGATACCCGGTATTACGATTTTTGGAGTGGTTATTATCCGCAAAGAGGACCAGGGCAGTCCTACTACTACTACGGTATGGACGTCTGG CARGDTRYYDFWSGYYPQRGPGQSYYYYGMDVW IGHV3-11*04 IGHD3-3*01 IGHJ6*02 IGHM assemble41 0
1 1.041667e-02 TGTGTGAGAGGGGCCATCCCCTACGGTGAGTAAACACGAATATTACTACTACTACTACATGGACGTCTGG out_of_frame IGHV3-52*01 IGHD4-23*01 IGHJ6*04 IGHM assemble60 0
1 1.041667e-02 TGTGCGAGAGACCCCTATTACTATGATAGTAGTGGTTATATGCCGTGACCCAGCTCGACCCCTGG out_of_frame IGHV4-61*01 IGHD3-22*01 IGHJ5*02 . assemble79 0
1 1.041667e-02 TGTGCGAGGACCGGCCCTATAGCAGTGGCTGGTTTCACTAGGTTACTGG out_of_frame IGHV1-46*01 IGHD6-19*01 IGHJ4*02 . assemble77 0
1 1.041667e-02 TGTGCGAGAGATGAGATTACGATATTTTGACTGGTTATTATCTGACCGAGCTGGTTTTGAGTTCCCTGAAATCCACCTAACCTGCGCAACTACTGG CARDEITIF_LVII_PSWF_VP_NPPNLRNYW IGHV1-3*01 IGHD3-9*01 IGHJ4*02 . assemble57 0
1 1.041667e-02 TGTGCGAACGGAGGCAGCAGAAAGGTGTCTTGGGATGGTTCGACCCCTGG out_of_frame IGHV3-NL1*01 IGHD3-10*01 IGHJ5*02 . assemble53 0
1 1.041667e-02 TGTGCGAGAGACTTAGCAGTGGCTCCCCCGGCTAGTACTACTTTGACTACTGG out_of_frame IGHV1-2*04 IGHD4-17*01 IGHJ4*02 IGHM assemble7 0
1 1.041667e-02 TGTGCGAGAGATGGAAGCGGCGTATTACGATTTTTGGAGTGGTTCTTCCGACCATACTACTACTACTACATGGACGTCTGG CARDGSGVLRFLEWFFRPYYYYYMDVW IGHV3-11*04 IGHD3-3*01 IGHJ6*04 IGHM assemble67 0
1 1.041667e-02 TGTGCGAGAGACCTCGGCCGTAGGTATAGCAGTGGCTGGTTCTGTTTCGACCCCTGG CARDLGRRYSSGWFCFDPW IGHV3-71*01 IGHD6-19*01 IGHJ5*02 . assemble17 0
1 1.041667e-02 TGTGCGAAAGATATACTAGTATGATAGTAGTGGTTATGGGTTCATTGTTTACTACTGG out_of_frame IGHV3-NL1*01 IGHD3-22*01 IGHJ4*02 IGHM assemble75 0
1 1.041667e-02 TGTGCGAGAGCTGTAACTTATATTGTAGTAGTACCAGCTGCGGGCTGACAGTTTACTACTACTACTACGGTATGGACGTCTGG out_of_frame IGHV1-3*01 IGHD2-2*01 IGHJ6*02 IGHM assemble50 0
1 1.041667e-02 TGTGCGAAAGATAGTAGTGGCTACGATTGAATGTTGACTACTGG out_of_frame IGHV3-NL1*01 IGHD5-12*01 IGHJ4*02 . assemble58 0
1 1.041667e-02 TGTGCGAGAGACCGGCGTTCGTGATACAGCTATGGTTACCGGACACTCCTACTACTACATGGACGTCTGG out_of_frame IGHV1-18*01 IGHD5-18*01 IGHJ6*04 IGHM assemble49 0
1 1.041667e-02 TGTGCGAGAGATCGGAGATACCCCTTGACAAGGTATAGCAGTGGCTGGTACGAAATTAATGATGCTTTTGATATCTGG CARDRRYPLTRYSSGWYEINDAFDIW IGHV3-11*01 IGHD6-19*01 IGHJ3*02 . assemble69 0
1 1.041667e-02 TGTGCGAAAGATCTCCCGAACTTATGTATATGTCTGGTGGTTCGACCCCTGG out_of_frame IGHV3-NL1*01 IGHD2-8*02 IGHJ5*02 IGHM assemble56 0
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