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Main projects during my current position in Petr Baranov Lab | Schepens Eye Research Institute, Mass General Hospital, Harvard Medical School

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Controlling Donor and Newborn Neuron Migration and Maturation in the Eye Through Microenvironment Engineering

33199396-01FA-4906-8954-B91C86AF4243

In this repository you may find the information for reproducing the bioinformatical analysis of human fetal, organoids and adult retina datasets.

The analysis in this study includes:

  1. R-Seurat datasets processing and de novo annotation
  2. GSEA pathway analysis
  3. Migration patterns search
  4. Python-ForceAtlas2 transformation (scanpy, scFates) and migration-maturation correlation

Files for reproducing the analysis: retina_singlecell.R, retina_scfates.ipynb, RGC_fetal_merged.ipynb.

The processed datasets are available here.

For any questions, suggestions and ideas, send an e-mail to:

  1. Jonathan R Soucy, [email protected], corresponding author
  2. Petr Baranov, [email protected], corresponding author, PI
  3. Emil Kriukov, [email protected], bioinformatics

Paper preprint is available on bioRxiv.