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Updating based on PR suggestions from LilyAnderssonLee
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Co-authored-by: Lili Andersson-Li <[email protected]>
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atrull314 and LilyAnderssonLee authored Sep 11, 2024
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Expand Up @@ -33,7 +33,7 @@ On release, automated continuous integration tests run the pipeline on a full-si
2. Unzip and split FASTQ ([`pigz`](https://github.com/madler/pigz))
1. Optional: Split FASTQ for faster processing ([`split`](https://linux.die.net/man/1/split))
3. Trim and filter reads. ([`Nanofilt`](https://github.com/wdecoster/nanofilt))
4. Post trim QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/), [`NanoPlot`](https://github.com/wdecoster/NanoPlot) and [`ToulligQC`](https://github.com/GenomiqueENS/toulligQC))
4. Post trim QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/), [`NanoPlot`](https://github.com/wdecoster/NanoPlot), [`NanoComp`](https://github.com/wdecoster/nanocomp) and [`ToulligQC`](https://github.com/GenomiqueENS/toulligQC))
5. Barcode detection using a custom whitelist or 10X whitelist. [`BLAZE`](https://github.com/shimlab/BLAZE)
6. Extract barcodes. Consists of the following steps:
1. Parse FASTQ files into R1 reads containing barcode and UMI and R2 reads containing sequencing without barcode and UMI (custom script `./bin/pre_extract_barcodes.py`)
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