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Workflow

luissian edited this page Mar 25, 2019 · 7 revisions

When the investigator starts the sample preparation in the lab, until the information is displayed on the iSkyLIMS the run is going through several steps.

The following picture shows a summary of these steps for a NextSeq run.

Sample preparation

During this phase the investigator is responsible to prepare the samples, the used library kits.

Create Sample -sheet

Investigator has to prepare the sample sheet that will be used later on for the wetlab Manager to create the run in iSkyLIMS.

The safest way to prepare the file is using the illumina software (IEM). With this tool the investigator will select the library kit used in his experiment and in will assign the indexes for each cell.

Check the Investigator role for more information about this file.

When saving the sample sheet file the investigator will delivery it to the wetlab Manager

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